藏药如意珍宝丸的质量标准研究.pdfVIP

国际中医中药杂志2017年1月第39卷第1期 Int J Trad Chin Med, January 2017, Vol. 39, No.1                      ·53 ·  ·实验与方药 · 藏药如意珍宝丸的质量标准研究 韩亚南 袁铭 周娟 汪娟 【摘要】 目的 建立藏药如意珍宝丸的质量标准。方法 采用薄层色谱法对诃子、降香、木香、 沉香、荜茇、丁香、高良姜、红花、肉桂、决明子、乳香、人工牛黄 12 味中药进行鉴别，采用 HPLC 法 测定没食子酸、去氢二异丁香酚、胡椒碱、桂皮醛含量。结果 12 味中药薄层色谱鉴别中斑点清晰，分 离度好；测定没食子酸在 0.106～0.901 μg、去氢二异丁香酚在0.033～0.281 μg、胡椒碱在0.007～0.060 μg、 桂皮醛在 0.021～0.178 μg 范围内线性关系良好，平均回收率在 98.47%～101.65%，RSD ＜3.0% 。结论 所 建立的鉴别和含量测定方法专属性强，准确性和重复性好，可用于如意珍宝丸的质量控制。 【关键词】 色谱法，薄层；色谱法，高压液相；质量控制；如意珍宝丸 Establishment of quality standard for Tibetan medicine Ruyi-Zhenbao pill Han Yanan, Yuan Ming, Zhou Juan, Wang Juan. Beijing Traditional Tibetan Hospital, Beijing 100029, China Corresponding author: Wang Juan, Email: wangshe199@126.com 【Abstract 】 Objective To establish a quality standard for Tibetan medicine Ruyi-Zhenbao pill. Methods Thin-layer chromatography (TLC) was applied to identify 12 Tibetan medicinal materials, and High performance liquid chromatography (HPLC) was employed to determine the contents of gallic acid, dehydrodiisoeugenol, piperine, and cinnamaldehyde. Results The identified characteristics of TLC were distinct and the spots were clear. Linearity of gallic acid, dehydrodiisoeugenol, piperine, and cinnamaldehyde were in the range of 0.106-0.901 μg, 0.033-0.281 μg, 0.007-0.060 μg, and 0.021-0.178 μg, respectively. Average recovery was in the range of 98.47%-101.65% (RSD3.0%). Conclusions The method of identification and content determination was good in terms of specificity, accuracy and repeatability, and can be used for quality control of Ruyi-Zhenbao pill. 【Key words 】 Chromatography, thin layer; Chromatography, High pressure liquid; Quality control; Ruyi-Zhenbao pill 如意珍宝丸是由诃子、降香、木香、沉香、 制剂质量控制。按我国药品质量管理的要求，任 荜茇、丁香、