毛细管电泳法测定牛乳中_乳白蛋白含量的研究.pdf

DOI:10.13684/j.cnki.spkj.2010.10.016 食品科技 2010 年第35 卷第 10 期 FOOD SCIENCE AND TECHNOLOGY 食品安全与检测 毛细管电泳法测定 牛乳中α-乳白蛋白含量的研究 孙国庆， 康小红， 刘卫星 ( 内蒙古蒙牛乳业(集团)股份有限公司研发中心，呼和浩特011500) 摘要： 将样品经样品缓冲液处理，毛细管柱选择为直径75 μm ，有效长度600 mm 的未涂层毛细 管柱，柱温40 ℃ ，缓冲溶液为柠檬酸缓冲液(pH 为3.0±0.2) ，工作电压为27 kV ，检测波长 为214 nm ；进样时间为5 s ，进行检测。结果α-乳白蛋白平均回收率为80%～110%，相对标准 偏差(RSD)为0.17%～2.8% ，最低检出限为0.005 mg/mL。该方法简便，准确，灵敏度高，是快速检 测牛乳中α-乳白蛋白含量最佳分析方法。 关键词： 毛细管电泳法；α-乳白蛋白；牛乳 中图分类号： TS 252.7 ；O 657.8 文献标志码： A 文章编号： 1005-9989(2010)10-0273-03 Detection of α-lactalbumin in bovine milk by capillary electrophoresis SUN Guo-qing, KANG Xiao-hong, LIU Wei-xing (Inner Mongolia Meng Niu Dairy Industry(Group) Co.,Ltd, RD Center, Huhhot 011500) Abstract: The samples were handled by the sample buffer, use a capillary that the diameter was 75 μ m and the effective length was 600 mm, capillary temperature was 40 ℃, buffer solution was citric acide buffer (pH of 3.0 ±0.2), separated voltage was 27 kV, detection wavelength was 214 nm, injection time was 5 s. As the results, the average recoveries ranged from 80%～110% and the relative standard deviation (RSD) was 0.17%～ 2.8% for α -lactalbumin. The limit of detection is 0.005 mg/mL. The present method is simple, accurate and sensitive. It is suitable for fast screening and a quantitative tool for monitoring of the α -lactalbumin in bovine milk. Key words: capillary electrophoresis(CE); α-lactalbumin(α-La); bovine milk α-乳白蛋白(α-lactalbumin)是牛乳特异蛋白