SectionJ-克隆DNA的分析与应用.pptxVIP

Section J Analysis and use of cloned DNA;J1 Characterization of clones J2 Nucleic acid sequencing（测序） J3 Polymerase chain reaction（PCR） J4 Organization of cloned genes J5 Mutagenesis（诱变） of cloned genes J6 Application of cloning;Restriction mapping（限制图谱） Sequencing (DNA RNA) （测序） Northern and Southern blotting PCR;J1 Characterization of clones;J1-1 Characterization;Size of DNA fragment cloned;J1-2 Restriction Mapping;1. Combinational enzyme digestion;2. Partial digestion;*;J1-4 Labeling of DNA or RNA probes ;End labeling;J1 Characterization of clones;J1-5 Southern and Northern blotting;Sou