HPLC检测大鼠血浆中莫西沙星浓度与其药动学研究.PDFVIP

HPLC 检测大鼠血浆中莫西沙星浓度及其药动学研究 朱加银a b c b b* ，阚歆 ，楼旦 ，董洁 ，胡国新 (温州医学院，a.实验动物中心；b.药学院；c.附属第二医院，浙江 温州 325035) 摘要：目的 建立HPLC 测定大鼠血浆中的莫西沙星浓度的方法，并进行体内药动学研究。方法 血浆样品采用二氯甲 烷液-液萃取法提取。色谱柱为 ZORBAX Edipse XDB-C8(150 mm ×4.6 mm ，5 μm) ，流动相为水-0.1%三氟乙酸-乙腈 1 ，检测波长296 nm ，柱温30 ℃。结果 莫西沙星浓度在0.05~10.00 μg·mL1 内线性关 (40 ∶35 ∶25) ，流速1.0 mL·min 系良好(r=0.999 6) ；最低定量限为 0.05 μg·mL1 ；低、中、高浓度的方法回收率为 99.3%~106.0% ，提取回收率为 88.1%~94.1%；日内、日间精密度RSD 均10% 。在大鼠体内药动学过程符合二室模型。结论 方法简便、经济、准确，可 满足临床药动学研究的要求。 关键词：莫西沙星；高效液相色谱法；血浆 中图分类号：R945.1; R917.101 文献标志码：A 文章编号：1007-7693(2011)08-0759-04 Determination of Moxifloxacin in Rat Plasma by HPLC and Its Application in Pharmacokinetic Study a b c b b* ZHU Jiayin , KAN Xin , LOU Dan , DONG Jie , HU Guoxin ( Wenzhou Medical College, a.Laboratory Animal Center; b.School of Pharmacy; c.the Second Affiliated Hospital, Wenzhou 325035, China) ABSTRACT: OBJECTIVE To establish an HPLC method for the determination of moxifloxacin in rat plasma, and validated for pharmacokinetic study. METHODS The moxifloxacin was extracted from plasma by dichloromethane. Chromatographic separation was carried out on ZORBAX Edipse XDB-C8 column(150 mm ×4.6 mm, 5 μm) with water-0.1% trifuoroacetic acid-acetonitrile(40 ∶35 ∶25) as the mobile phase at a flow rate of 1.0 mL·min1. The detection wavelength was 296 nm and the column temperature was 30 ℃. RESULTS The linear range was 0.0510.00 μg·mL1(r=0.999 6). The lowest detectable limit was 0.05 μg·mL1. The recovery of the method was 99.3%106.0%, and the extraction recovery was 88.1%94.1%. RSDs of inter- and intra-day precisions were both less than 10%. The profile of moxifloxacin in vivo fitted a two-compartment model. CON