水飞雄黄和粗颗粒雄黄中三氧化二砷的含量测定精选.docVIP

水飞雄黄和粗颗粒雄黄中三氧化二砷的含量测定 任建锋，龚千锋*，易炳学，王丕明，朱龙涛，余香 （江西中医学院，江西 南昌330004） 摘要:目的：建立水飞雄黄和粗颗粒雄黄中三氧化二砷的含量测定方法。考察水飞雄黄和粗颗粒雄黄中三氧化二砷的含量变化。方法：以研磨成糊状的加水量为3ml，总的用水量为3000ml，干燥温度为40℃，干燥时间为2h。恒温干燥箱为加热设备，得到炮制品；研钵研磨粉碎，粉碎成粒径20目左右的颗粒（粗粉），采用紫外分光光度法测定雄黄中可溶性砷盐As(Ⅲ)的含量。检测波长510nm。结果：粗颗粒雄黄中三氧化二砷的含量较水飞雄黄中的含量低，另外随着炮制干燥温度的不断升高，水飞雄黄中三氧化二砷的含量有所提高。且三氧化二砷在4-20ug范围内线性关系良好，r=0.9995；平均回收率为101.5﹪；RSD为2.96﹪。结论：方法简便、快速、准确可靠、重复性好，可为水飞雄黄和粗颗粒雄黄药材的质量控制提供参考。 【关键词】：水飞雄黄；粗颗粒雄黄；三氧化二砷；紫外分光光度法 The water flies in the realgar and the coarse particles realgar the white arsenic content determination REN Jian-Feng，GONG Qian-Feng，WANG Pi-Ming，ZHU Long-Tao，YU Xiang （Jiangxi University of Traditional Chinese Medicine,Jiangxi Nanchang 330004） Abstract:Objective: The establishment water flies in the realgar and the coarse particles realgar the white arsenic content determination method. Investigation water fly Realgar and coarse particle impurities in petroleum-derived arsenic trioxide content changes.Methods: as grinding into paste water dose for3ml, total water dose for3000ml, dryness temperature 40℃and dryness time 2h , Research bo grinding shattered, Shattered into about 20 mesh size of particles (thick powder).The content of dissolve darsenic As(Ⅲ) in Realgar was tested by ultraviolet spectrophotometric method, detection wavelength 510nm.Results: coarse particle Realgar of As2O3 than the processed products to decreased a certain extent, the other with ever increasing processing temperature, Realgar of processed products of As2O3 content enhance.And As2O3 in the range of 4-20ug a good linear relationship, r = 0.9995; The average recovery was 101.5%; RSD was 2.96%.Conclusion: he method is simple, rapid, accurate and reliable, reproducible and Realgar medicines for water fly Realgar and coarse particle Realgar quality control for reference. Key words：The water flies in the realgar; Coarse particle Realgar ;As2O3; ultraviolet spectrophotometric method 28种毒性药材之一[2]。雄黄其性状为块状或粒状结合体，呈不规则块状。深红色或橙红色，条痕淡橘红色，晶面有金刚石样光泽。质脆，易碎，断面具树脂样光泽