罗库溴铵对大鼠正常或损伤面神经与肢体神经肌接头突触前膜乙酰胆碱量子式释放的影响.pdfVIP

T he effect of rocu ron ium on presynaptic quantal release of acety lch olin e from n orm al or dam aged facial nerve an d som atic n erve inn ervated n eu rom u scu lar junction s in rats A b str a ct O bjective： T o com pare the am ount of presynaptic quantal release of acety lcholine from norm al or dam aged facial nerve and som atic nerve innervated neurom uscular junctions under the effect of different concentrations of rocuron ium in rat in vitro. T o evaluate w hether the differences in sensitiv ity to rocuron ium am ong the norm al or dam aged facial nerve and som atic nerve innervated m uscles are related to the function of presynaptic neurotran sm itter release. M ethods： A utologous paired controlled design w as used in the study. A n acute nerve trunk squeeze injury m odel w as induced in the right facial nerves of the rats and the left fac ia l n erv e s w ere u sed fo r co n tro l. T h e facia l n erv e tru nk s from b o th sid e s w ith the connected orbicu laris oris m uscle strips and the som atic nerve trunks w ith the conn ected gastrocnem iu s m uscle strip s w ere isolated for m easuring the endplate potentials (E PP) and the m iniature endplate potentials (M EPP) from the three groups of neurom uscular junctions by using intracellular m icroelectrode gauge. T he EP P s and M E PP s w ere recorded at different concentrations of rocuron ium (2.5，5, 7.5, lOjig /m l)，and then, the presynaptic quantal release of acetylcholine w hich w as calculated from the values of E PP/M EPP w as com pared am ong norm al or dam aged facial nerve and som atic nerve inn ervated neurom u scu lar junction s. R esu lts： T he E PP s and M E P P s from all the three group s of neurom u scu lar junctions exhib ited a dose-dependent decreasing as the concentration of rocuronium increased. A t rocuronium concentrations of 2 .5，5, 7.5 and 10pg/m l，the extent of decreasing in