高效液相色谱法在甘油含量测定中应用研究.pdfVIP

∀ 646 ∀ 2007 , , ( , 200135) [1] : 现有的甘油含量测定标准化方法, 都是以假设 无杂质 为基础的该法与气相色谱法 , 以分离色谱为基础, 解决了滴定法不具分离鉴别的缺点完善了原气相色谱法在不同含量规格 [1] 适用范围上的局限性 , 使液相色谱法对不同含量规格甘油定量测试的方法标准化成为可能该 法与气相色谱的 杂质倒扣 法的结合应用和互补, 使定性鉴别和不同规格甘油含量测定得以完整 组合其检测下限: 44. 46 ng/ L, 线性相关系数r= 0. 99999, 回收率: 98. 03% ~ 99. 88%, 重复性: Sr = 0. 4018, r 95 = 1. 125, 再现性: SR = 0. 4463, R95 = 1. 250 : 甘油( 丙三醇) ; 高效液相色谱法; 示差折光检测器; 含量测定 : O 657. 7; TQ 223. 16 :A : 0367 6358( 2007) 11 646 04 A lication of HPLC Method in Determining Content of Glycerol SUN Dun wei, WU Xiao hong, ZHANG Lin ( Shanghai Entry exit Inspection and Quarantine Bureau, Shanghai 200135, China) Abstract : Current standard methods for determining the content of glycerol are all based on assuming the determined glycerol to have no im urity exce t water. The methods of HPLC and GC[ 1] in glycerol determination are all based on chromatogra hic fractionation. Therefore, they can overcome the defects of chemical titration method on qualitative identification and im urity se aration. This HPLC methodwill further erfect the limitation that reviously existed in GC [ 1] standard method on the a licable range of different content s ecifications, and make it ossible to standardize the test method for the different content s ecifications of glycerol by HPLC method. It will be a com lete combination for qualitative identification and quantitative analysis for the different content s ecifications of glycerol by the combined a lication and mutual com lement of these HPL