黄瓜枯萎病防治药剂的离体和活体筛选.pdfVIP

中国蔬菜 2010（12）：60-65 CHINA VEGETABLES 黄瓜枯萎病防治药剂的离体和活体筛选 1，2 2 2 2 2* 段广荣 石延霞 谢学文 王微微 李宝聚 1 2 （沈阳农业大学植物保护学院，辽宁沈阳 110161； 中国农业科学院蔬菜花卉研究所，北京 100081） 摘 要：选取蔬菜上常用的 17 种杀菌剂，运用离体抑菌活性筛选和药剂拌土盆栽活体筛选两种方法， 测定了供试药剂对黄瓜枯萎病的生物活性。离体抑菌活性测定结果表明，抑菌活性较高的为己唑醇、氟硅 唑、腈菌唑、福美锌、福美双、代森锰锌、嘧霉胺、甲基硫菌灵、多菌灵，其抑菌率在 69.61 %～100.00 % 之间；盆栽活体测定结果表明，嘧菌酯、醚菌酯、烯肟菌酯、嘧霉胺、多菌灵、甲基硫菌灵的防效在 61.50 %～95.90 %之间，可作为土壤处理剂防治黄瓜枯萎病。 关键词：黄瓜枯萎病；杀菌剂；药剂拌土；活体筛选；离体筛选 中图分类号：S436.5 文献标识码：A 文章编号： 1000-6346（2010）12-0060-06 Fungicides in Vitro and in Vivo Screening against Fusarium oxysporum f. sp. cucumerinum 1,2 2 2 2 2* DUAN Guang-rong , SHI Yan-xia , XIE Xue-wen , WANG Wei-wei , LI Bao-ju 1 2 （College of Plant Protection, Shenyang Agricultural University, Shenyang 110161, Liaoning, China; Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China ） Abstract ：Seventeen common fungicides were chosen to screen the effectiveness of cucumber （Cucumis sativus L.）against Fusarium wilt in vitro and in vivo. The results of antibacterial activity assay in vitro showed that the antibacterial activity of hexaconazole, flusilazole, myclobutanil, ziram, thiram, mancozeb, pyrimethanil, thiophanate-methyl and carbendazim was higher, and the inhibition rate in vitro was between 69.61 %-100.00 %. Potted test in vivo showed that the anti-effect of azoxystrobin, kresoxim-methyl, enestroburin, pyrimethanil,