利用种子蛋白 SDS－PAGE 技术进行抗热萝卜种质鉴定.pdfVIP

中国蔬菜 2010（8）：21-25 CHINA VEGETABLES 利用种子蛋白 SDS －PAGE 技术进行抗热萝 卜种质鉴定 * 朱长志 赖德强 吴 斌 张彦玉 柳李旺 （南京农业大学作物遗传与种质创新国家重点实验室，南京农业大学园艺学院，江苏南京 210095） 摘 要：运用 SDS 聚丙烯酰胺电泳（SDS）技术，对 32 份抗热萝卜材料种子蛋白的遗传多态性 进行研究，共检测出 41 条蛋白谱带，多态性谱带 25 条（61 %）；每份材料可检测出 28～39 条谱带，所有 材料蛋白谱带均有差异，可进行品种鉴别；供试材料蛋白谱带相似系数为 0.64～0.98，平均为 0.79；聚类分 析表明，在相似系数 0.76 处所有种质材料可分为白皮与红皮两大类。研究结果显示，基于种子蛋白谱带的 聚类结果与肉质根皮色有较高的相关性，抗热萝卜材料遗传多样性较低，遗传基础比较狭窄。 关键词：萝卜；种子蛋白；SDS；种质鉴定 中图分类号：S631.1 文献标识码：A 文章编号： 1000-6346（2010）08-0021-05 Using Seed Protein SDSto Identify Heat-resistant Radish Germplasm * ZHU Chang-zhi，LAI De-qiang，WU Bin，ZHANG Yan-yu，LIU Li-wang （National Key Laboratory of Crop Genetics and Germplasm Enhancement ，College of Horticulture ，Nanjing Agricultural University ，Nanjing 210095 ，Jiangsu ，China ） Abstract ：Seed proteins of 32 heat-resistant radish（Raphanus sativus L.）accessions were analyzed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis （ SDS） to estimate their polymorphism．A total of 41 protein bands were detected，of which 25（61 %）were polymorphic．Each accession could produce 28 to 39 protein bands，their profiles were different with each other and all accessions could be distinguished．These accessions could be clustered into two major groups of white and red roots at similarity index of 0.76 with UPGMA．The protein band similarity coefficient ranges from 0.64 to 0.98 and the average is 0.79．The results revealed that cluster analysis of seed protein was highly in accordance with radish root color，and low genetic diversity