响应面优化蜂花粉可溶性膳食纤维提取工艺.pdfVIP

2015 年 2 月第 35 卷第 2 期 湖南中医药大学学报 20 Journal of Hunan Univ. of CM Feb. 2015 Vo l. 35 No. 2 ·中药制剂与分析· 响应面优化蜂花粉可溶性膳食纤维提取工艺 郑 慧，陈希平*，常新利，王L德风，陈珍珍，陈 占百 (湖南中医药大学药学院，湖南长沙 410208) 〔摘要〕为充分利用蜂花粉资源，采用酸提工艺提取蜂花粉可溶性膳食纤维，利用单因素试验和响应面优化法对液料比、浸 提 pH 值、浸提温度、浸提时间工艺条件进行分析与优化，并测定蜂花粉可溶性膳食纤维的理化指标。结果表明，蜂花粉可溶性 膳食纤维酸提最优工艺为:液料比 10.8: 1(mUg)、浸提 pH 值 4.3 、浸提温度 50 t:、浸提时间1.9 h ，在此工艺条件下蜂花粉可溶 性膳食纤维得率为 7.31% ，膨胀力 0.87 mUg、持水力1.33 g/g、水溶性 84.780毛。 〔关键词〕蜂花粉:可溶性膳食纤维;响应面优化;提取工艺 〔中图分类号)R284.2;TS218;Q949.94 (文献标识码)B (文章编号)doi: 10.3969/j.issn.1674-070X.20l 5.02.007.020.05 Extraction Process Optimization for Soluble Dietary Fiber from Bee Pollen by Response Surface Methodology ZHENG Hui , CHEN Xiping飞 CHANG Xinli , KONG Defeng, CHEN Zhenzhen, CHEN Juαn (School of Pharmacy, Hunan Universιty of Chinese Medici肘， Chαngsha， Hunan 410208, China) (Abstract) In order to fully utilize bee pollen resource , the soluble dietary fiber from bee pollen was extracted using acid extraction process. The process conditions of liquid-to-solid, extraction pH , extraction temperature , extraction time were analyzed and optimized by single-factor experiment and response surface methodology. The physicochemical properties of bee pollen soluble dietary fiber were measured. The optimal extraction conditions were determined as follows 10.8: 1 (mUg) of liquid -to -solid, pH4.3 , extraction temperature 50 t:, extraction time 1.9 h. Under the conditions , the yield of bee pollen soluble dietary fiber was 7.310毛， swelling capacity was 0.87 mUg, water holding capacity was 1.33 g/g , and the water solubility was 84.78%. (Keywords) bee pollen; soluble dietaηfiber; 而sponse surface optimization; extraction process