响应面法优化海洋枯草芽孢杆菌HS-A38增殖发酵培养基.pdfVIP

第 31 卷第 1 期 大连工业大学学报 Vo l. 31 No.l 201 2 年 1 月 Journal of Dalian Polytechnic University ]an. 2 0 1 2 文章编号:1674-1404(2012)01-0019-05 响应面法优化海洋枯草芽抱忏菌 HS-A38 增殖发酵培养基 张 丹，谢三群 欢，丛丽娜，侯英敏，王 (大连工业大学生物工程学院，辽宁大连 116034 ) 摘要:在摇瓶培养条件下，优化提高海洋枯草芽抱杆菌菌体浓度的发酵培养基组分。采用 Plackett­ Burman 法确定了影响菌体浓度的显著性因子，即葡萄糖和 ZnSO，;通过最陡爬坡实验逼近这两个重要 因子的最大响应稳定区域，采用中心组合实验确定显著性因子的最佳水平，并用 Design expert 7. 13 软 件进行回归分析。优化后培养基的组成为:葡萄糖 8.49 g/L ，豆粉粉 12.0 g/L，尿素 0.625 g/L ，酵母膏 4. 0 g/L ,K HPO , 4. 0 g/L ,ZnSO, O. 053 g/L。优化后的活菌浓度达到1. 64 X 10 cfu/mL，与优化前菌 2 体浓度(7.22 X 108 cfu/mL)相比，提高了 1 倍多。通过统计优化培养基组分可有效提高海洋枯草芽抱 杆菌 HS-A38 的发酵液菌体浓度。 关键词:枯草芽抱杆菌;响应面法;活菌数F 优化 中固分类号: Q939.97 文献标志码:A Medium optimization for spore production of marine Bacillus subtilis HS-A38 by response surface methodology ZHANG Huan , CONG Li-na , HOU Ying-min , WANG Dan , XIE San-qun ( School of Biological Engineering , Dalian Polytechnic University , Dalian 116034 , China) Abstract: U sing shaking-flask fermentation in the laboratory , glucose and ZnSO, affecting spore production of marine Bacillus subtilis HS-A38 were determined by the design of Plackett-Burman. The region of the fermentation conditions was approached through the path of steepest ascent. Subsequently , the concentration of those important factors were optimized by Central Composite Design (CCD) and response surface analysis by Design expert 7.13. The concentration of ingredients is as glucose 8.49 g/L , soybean powder 12.0 g/L , urea O. 625 g/L , yeast extract 4.0 g/L , K2 HPO, 4. 0 g/L , ZnSO, 0.053 g/ L. The colony-forming units per m