响应面法优化玉米芯木聚糖提取条件.pdfVIP

河南农业科学.2012.41(2):157-160 Journal of Henan Agricultural Sciences 晌应面法优化玉米芯木聚糖提取条件 华承伟1 ，谢凤珍2 ，陈晓静2 (1.洞南科技学院生命科技学院，商南新乡 4530031 2. 河南科技学院新科学院，何南新乡 453003) 摘要z 为提高玉米芯中木聚糖得率，采用响应面法对碱法提取玉米芯木聚糖条件进行优化。利用 Oesign - Expert 8 的 Min Run Res N 多因子两水平设计，对影响木聚糖提取的因素进行效应评价， 利用最哨攀登搜索法为响应面分析的中心组合设计确定中心区，利用响应面分析得到优化提取条 件e 结果表明，碱提温度、碱提时间、NaOH 质量分数和料淡比对木聚糖提取率有显著影响，其最 佳条件为:NaOH 质量分数16%. 温度85 c.料液比 1 : 16.提取时间 2.5 h. 利用响应面法成功对 玉米芯木聚糖提取条件进行优化，提取率达到 25.6%。 关键词z 玉米芯F 木聚糖p 提取条件g 响应面$优化 中固分类号: Q503 文献标志码:A 文章编号: 1004 - 3268(2012)02 - 0157 - 04 Optimization of Extraction Conditions of Xylan from Corn Cob by Response Surface Methodology HUA Cheng-wei1 .XIE Feng-zhen2 .CHEN Xiao-jing2 O. Sch∞1 of Life Science and Technology ,Henan Institute of Science and Technology ,Xinxiang 453003 ,China; 2. Xinke College , Henan Institute of Science and Technology ,Xinxiang 453003 ,China) Abstract: The conditions of alkali extraction for increasing xylan yield from corn cob were opti- mized by response surface methodology (RSM). Min Run Res N design of Oesign-Expert 8 was used to evaluate the influence of different factors. and then the steepest ascent path was employed to determine the central region of the medium composition for the further central composite de- sign (CCO). Four factors which had significant effects were screened out.including temperature , time.concentration of NaOH and the ratio of solid to liquid. The optimal composition of these fac- tors were 16% of NaOH , 85 C , liquid/material ratio of 1 I 16 , and 2. 5 h by RSM. Under the above conditions ,the yield of corncob xylan was up to 25.6%. Key words: corn cob; xylan; extraction conditions; response surface methodology;