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Journal of Pharmaceutical and Biomedical Analysis xxx (2006) xxx–xxx Chemical characteristics for different parts of Panax notoginseng using pressurized liquid extraction and HPLC-ELSD J.B. Wan a , F.Q. Yang a , S.P. Li a,∗, Y.T. Wang a , X.M. Cuib a Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau, China b Wenshan Prefecture Sanqi Research Institute, Wenshan, Yunnan, China Received 11 December 2005; received in revised form 28 January 2006; accepted 30 January 2006 Abstract The chemical characteristics for different parts of Panax notoginseng, including root, fibre root, rhizome, stem, leaf, flower and seed, were determined using high performance liquid chromatography-evaporative light scattering detection (HPLC-ELSD) and pressurized liquid extraction (PLE). Eight major saponins, namely notoginsenoside R1, ginsenosides Rg1, Re, Rb1, Rc, Rb2, Rb3 and Rd were also quantitatively compared among the different parts of P. notoginseng. The chromatograms showed that there was significant difference between underground (root, fibre root, rhizome) and aerial (leaf and flower) parts from P. notoginseng, though the similarities of entire chromatographic patterns among tested samples from underground (0.965 ± 0.029, n = 12) and aerial parts (0.987 ± 0.014, n = 5) were similar, respectively. Especially, no saponin was detected in the seed of P. notoginseng. Hierarchical clustering analysis based on eight investigated saponins or the ratios of contents for ginsenoside Rg1/Rb1 and ginsenoside Rb3/Rb1 showed that the samples from different parts of P. notoginseng were divided into three main clusters. One cluster was underground parts, which contained rich protopanaxatriol and protopanaxadiol types saponins. The leaf