混合菌种固体发酵菜粕生产氨基酸肥料的条件研究.pdfVIP

中国农业科学 2009,42(10):3530-3540 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2009.10.019 混合菌种固体发酵菜粕生产氨基酸肥料的条件研究 王永红，冉 炜，张富国，杨兴明，徐阳春，沈其荣 （南京农业大学资源与环境学院/江苏省固体有机废弃物资源化利用高技术研究重点实验室，南京 210095 ） 摘要：【目的】通过响应曲面法优化以菜粕作为主要基质，两株产蛋白酶菌株嗜麦芽糖寡养单胞菌 Stenotrophomonas maltrophilia G12（FJ211222）和短小芽孢杆菌 Bacillus pumilus K11（FJ211221）混合菌 种固体发酵生产氨基酸肥料的发酵过程参数，为实际生产提供理论依据。【方法】利用响应曲面法（response surface methodology），中的 Plackett-Burman 设计和 Box-Behnken 设计完成整个优化过程，用 Mintab 15.0 软 件和 Design-Expert 7.0 软件分析试验数据。【结果】最终确定了麸皮添加量，基质起始含水量和起始 pH 为影响 固体发酵酶解过程的主要因素，得出固体发酵过程主要工艺参数：发酵周期6 d，麸皮添加量14.1%，基质含水量 54.4%，起始 pH 9.15，接种量 5.0%，菌液体积比为 1 ﹕1，每 24 h 翻抛 1 次。【结论】发酵过程参数优化后的菜 粕蛋白水解度达到了13.1%，增加了游离氨基酸和小肽含量。 关键词：氨基酸肥料；固体发酵；响应曲面法；水解度 Study on Conditions in Solid-State Fermentation of Rapeseed Meal by Mixed Strains for Amino Acid Fertilizer WANG Yong-hong, RAN Wei, ZHANG Fu-guo, YANG Xing-ming, XU Yang-chun, SHEN Qi-rong (College of Agricultural Resources and Environmental Sciences, Nanjing Agricultural University/Jiangsu Provincial Key Laboratory for Solid Organic Waste Utilization, Nanjing 210095 ) Abstract: 【Objective 】 An optimization of parameters in solid-state fermentation of rapeseed meal by mixture of two protease-producing bacterial strains Stenotrophomonas maltrophilia G12 ( FJ211222) and Bacillus pumilus K11 (FJ211221) for an amino acid fertilizer was studied to provide a theoretical basis in the industrial production. 【Method 】 The fermentation processes were optimized by using of Response Surface Methodology (RSM) with Plackett-Burman Experiments and Box-Behnken Design. Data were analyzed with Design-Expert 7.0 software and Mintab 15.0 software. 【Result 】 High amino acid production in ferme