大鼠血浆中人参皂苷化合物K的UPLC-MSMS法测定.pdfVIP

中国医药工业杂志 Chinese Joumal of Pharmaceuticals 2011 , 42 (9) • 683 • 大鼠血浆中人参皂苦化合物 K 的 UPLC-MS/MS 法测定 陈忠祥，陈亭亭，虞丽芳，杨耀杰，陆伟根* (中国医药工业研究总院药物制剂国家工程研究中心，上海 200437) 摘要2 建立了超高效液相色谱·串联质谱法测定大鼠血浆中的人参皂营化合物 K. 采用 BEH C8 色谱柱，以乙脯-0.1% 甲酸为流动相，梯度洗脱，以乙酸泼尼松龙为内标，负离子电喷雾离子化，多反应监测，药物和内标的监测离子对为 m/z667 → 161 和m/z447 → 329. 人参皂苦化合物K在2-2∞o ng/ml 浓度范围内线性关系良好，日内及日间 RSD 均小 子 10%. 关键词z 人参皂昔化合物K: 超高效液相色谱-串联质谱:测定 中图分类号: R917: 0657.7+2 文献标志码:A 文章编号: 1001-8255 (201 1)09-0683-04 Determination of Ginsenoside Compound K in Rat Plasma by UPLC-MS瓜.fS CHEN Zhixiang, CHEN Tingting, YU Lifang, YANG Yaojie , LU Weigen* (National Pharmaceulical Engineering Resea时Center， China State lnstitule 01Pharmaceutical lndus句~ Shanghai 20043乃 ABSTRACT: An UPLC-MSIMS method was established for the determination of ginsenoside compound K in rat plasma. A BEH C18 column was used with mobile phase of acetonitrile-O.l % formic acid by gradient elution. Prednisolone acetate was used as the intemal standard. Samples were detected by electrospray negative ionization mass spectrome町and multiple-reaction monitoring mode. The transitions of ginsenoside compound K and intemal standard were m/z 667.3• 16 1.3 and m/z 447.3 • 329.3. The calibration curve was linear in the concentration range of 2 - 2 000 nglml. The intra- and inter-day RSDs were less than 10%. Key Words: ginsenoside compound K; UPLC-MSIMS; determination 人参皂昔化合物K(ginsenoside compound K. 浆中的1，旨在为 1 的临床前及临床药动学研究提 1) 是人参中多种人参皂背在人肠道内的最终代谢产 供参考。 物[1]. 1 有抗炎、抗肿瘤、抗休克、降血糖、抗突 1 仪器与试药 ACQUITY 超高效液相色谱仪， Xevo TQ 三重四极杆质 变、抗皮肤衰老和抗过敏[2-9] 等多方面的药理活性。