大鼠血浆中利伐沙班的LC-MSMS法测定及其药动学.pdfVIP

• 722 • 中国医药工业杂志 Chinese Journal of Pharmaceuticals 2015 , 46(7) -~，~ .. g 药理与临床 3 ..,‘c c.c.c. ccc.c...‘c.c.C-- 大鼠血浆中利伐沙班的 LC-MS/MS 法测定及其药动学 崔文宁l.2 ，王淑梅l* ，杨秀岭\张志:青l ，王丽娜I (1.河北医科大学第二医院，河北石家庄 050000， 2. 河北省故城县医院，河北衡水 053800) 摘要z 建立了液相色谱-串联质谱法测定大鼠血浆中的利伐沙班(1)。采用 C8 色谱柱，以艾瑞昔布 (2) 为内标，乙睛: 10 mmol/L 乙酸绞溶液(用甲酸调至 pH 3.14) (50 : 50) 为流动相， ESI 源正离子检测方式，多反应监测，监测离子 对m/z 436.2• m/z 145.2 (1). m/z 370.1 • m/z 278.0 (2) 0 SO 大鼠经口灌胃给予1，主要药动学参数分别为: c ，， (438.19土 m 122.02)nglml, tmax (0.69士0.20)h， AUC _ (1692.75士654刀)ngh.mr ， AUC _ (1 760.25士649.11) nghmr , 1,12 (3.46:1::1.1 0) h。 o 24h o 关键词:利伐rJ、班:液相色谱-串联质谱:药动学:测定 中图分类号: 0657.γ2， R969 文献标志码:A 文章编号: 1001-8255 (2015) 07-0722-04 001: 10.16522/ki.cjph.2015β7.014 Determination ofRivaroxaban in Rat Plasma by LC-MS瓜1S and Its Pharmacokinetics CUI Wenningl WANG Shumei忡， YANG Xiuling t, ZHANG Zhiqingt, WANG Lina l 气 (1. The Second Hospital ofHebei Medical Universi职 Shijiazhuang 050000; 2. The Hospital ofGucheng County. Hebei Province, Hengshui 053800) ABSTRACT: An LC-MS!MS method was established for the determination ofrivaroxaban(l) in rat plasma. A CI8 column was used , with the mobile phase of acetontrile : 10 mmol/L ammonium acetate solution (adjustedωpH 3.14 by formic acid) (50 : 50) and imrecoxib (2) as the intemal standard. An ESI source was applied and operated in positive ion multiple-reaction monitoring mode. The detected ions were m/z 436.2• m/z 145.2 (1) and m々 370.1 →m々 278.0 (2). The main pharmacokinetic