大鼠血浆中盐酸多西环素的HPLC法测定.pdfVIP

• 762 • 中闰阪药工业杂志 Chínese Joumal of Phannaceuticals 2008 , 39 (10) 大鼠血浆中盐酸多西环素的HPLC法测定 罗华菲，张小缸，五雅珍，五浩* (上海医药工业研究院，药物制剂回家工程研究中心，上海 201203) 摘要2 建立了RP-HPLC法测定大鼠血浆中甜酸多西环素。以美他环素为内标，血浆样品用乙酸乙醋提取，采用C 色谱 I8 -80mmo1/L磷酸盐援冲液(pH 2.4)-磷酸·三乙胶(18 : 79.7: 0.5 : 1. 8. 调至pH 6.5) ，检测波长346nm. 桩，流动相为乙脯 监酸多西环素线饨范围为0.05-10μg!ml(r=O.9998) ，枪测限为0.04μg!ml。方法相对回收率为96.8% --104.2%. 绝对回收 率为82.4 %-92.8%; 日内RSD为3.6%-5.2% ，日问底SD为4.0%-7.6%。 关键诩t 盐酸多西环索1 血浆浓度:反相高效液相包谱:测定 中回分类号: TQ460.γ2; 0657.7+2 Jt献栋ì.R耐:A 文章编号: 1001-8255 (2008) 10-0762心3 Detennination of Doxycycline Hydrochloride in Rat Plasma by HPLC LUOHua布i， ZHANG Xiao-hong , WANG Ya-zhen , WANG Hao* (National Pharmaceutical Engineering and Rωearch Center, Shanghai Institute 01Pharmacω， ticallndus町\ Shanghaí 201203) ABSTRACT: A RP-HPLC method was established for the determination of doxycycline hydrochloride in rat plasma. Metacycline was used as intemal standard. The plasma samples were extracted with ethyl acetate. A C18 column was used with the mobile phase of acetonitrile幽80mmol/L phosphate buffer(pH 2.4)蝇phosphoric acid-triethylamine (1 8: 79.7 : 0.5 : 1.8, pH 6.5) , at the detection wavelength of 346nm. The calibration curve of doxycycline hydrochloride was linear in the concentration range of 0.05 - 10μglml(严0.9998). The detection Hmit w嗣 0.04μglml. The relatíve recove叩 was 96.8% 翩 104.2%， and the absolute recove叩 was 82.4% - 92.8 %. The RSDs of intra幽 and inter-daywere3.6% -5.2% and4.0% -7.6%. Key Words: doxycycline hydrochloride; plasma concentration; RP-HPLC; dete