小麦HMW-GS最佳测定时间的探讨及其应用.pdfVIP

中国农学通报 2009,25(17):15-19 Chinese Agricultural Science Bulletin 小麦HMW-GS 最佳测定时间的探讨及其应用 李彦美，王凤宝，付金锋，董立峰 (河北科技师范学院农学系，河北昌黎066600) 摘 要：为了探明小麦收获前测定高分子量谷蛋白亚基的最佳时间，实现高分子量谷蛋白亚基与综合育 种目标性状的同时选择。从小麦开花期始，对21 个小麦品种( 以中国春为对照)每5 天用SDS法 测定其HMW-GS 以寻找最佳测定时间，并对21 个杂交组合后代进行连年选择。结果表明，乳熟中期 HMW-GS 谱带始达清晰，是当代测定当代选择的最佳测定时期。通过连年高分子量谷蛋白亚基的选 择，部分高分子量谷蛋白优质亚基出现的频率得到显著的提高，选择效果良好。选育出高产优质小麦 新苗头品系宝麦35 ，证明当代测定高分子量谷蛋白亚基的同时结合综合育种目标性状进行选择，对于 选育优质，高产、抗逆新品种效果是良好的。 关键词：小麦；HMW-GS ；最佳测定时间；选择；利用 中图分类号：S512.1 文献标识码：A 论文编号：2009-1087 Discussion of Best Detected Period and Application of HMW-GS in Wheat Li Yanmei, Wang Fengbao, Fu Jinfeng, Dong Lifeng (Department of Agronomy, Hebei Normal University of Science Technology, Changli Hebei 066600) Abstract: In order to searching for the best detected period of HMW-GS before harvest of wheat, achieving the one time selected of HMW-GS and objective trait .The HMW-GS components of 21 wheat varieties were fractionated by SDSeach 5 days from anthesis to search for the best detected period ,and 21future generation of hybrid combination were continual selected. The resulted showed : （1）the mid-milky stage is the best stage to contemporary detection and selection which firstly has clear HMW-GS . （2）The some excellent HMW-GS frequency was improved according to continual selected of HMW-GS, the selected effect is good. （3）The new line Baomai 35 with high yield and quality were selected, proved that the selection which combines contemporary detected HMW-GS with breeding objective of comprehensive was benefit the new line selection of high yield and quality and resistance. Key words: wheat, HMW-GS, the best detected time, selection, application 0 引言