pIZTV5-His昆虫表达载体说明.pdfVIP

pIZT/V5-His 编号 载体名称 北京华越洋生物KCVECT106 pIZT/V5-His pIZT/V5-His 载体基本信息 载体名称: pIZT/V5-His 质粒类型: 昆虫表达载体；荧光报告载体 克隆方法: 多克隆位点，限制性内切酶 启动子: OplE2 载体大小: 3336 bp 5 测序引物及序列: OpIE2 Forward ：CGCAACGATCTGGTAAACAC 3 测序引物及序列: -- 载体标签: V5 Epitope Tag , His Tag (C-端) 载体抗性: Zeocin 真核筛选标记: Zeocin ；GFP 克隆菌株: TOP10 ；DH5alpha 宿主细胞（系）: Sf9, High Five pIZT/V5-His 载体含有荧光报告基因GFP ，与抗性基因Zeocin 融合表达， 备注: 即Zeocin-GFP 融合蛋白的形式；表达水平高。 瞬表达/稳表达: 瞬表达 组成型/诱导型: 组成型 病毒/非病毒: 非病毒 pIZT/V5-His 载体质粒图谱和多克隆位点信息 pIZT/V5-His 载体简介 The InsectSelect Glow Kit features the pIZT/V5-His vector for high-level expression of yourgene of interest. This vector has the following features: • The OpIE2 promoter for constitutive expression • The Zeocin resistance gene for rapid selection of stably transfected cell lines • C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Anti-V5 Antibody and rapid purification with nickel-chelating resin In addition, pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin resistance protein (Zeo-GFP). The Zeo-GFP fusion protein permits rapid selection of stably transfected cell lines with Zeocin and confers a fluorescent phenotype that simplifies identification of transfected cells. Promoter: OplE2 Vector Type: pIZ Product Size: 1 kit Cloning Method: Restriction Enzyme ⁄ MCS Protein Tag or Fusion: V5 Epitope Tag, His Tag (6x) Protein Tag Position (to your gene): C-terminal pIZT/V5-His 载体序列 ORI