白藜芦醇合成酶（RS）基因转化胡萝卜及高白藜芦醇转基因胡萝卜株系的筛选.pdfVIP

农业生物技术学报 Journal ofAgricultural Biotechnology 2007，15 (6)：987～991 · 研究论文· 白藜芦醇合成酶(RS)基因转化胡萝卜及 高白藜芦醇转基因胡萝卜株系的筛选，一c 林 明，曹 蕾，郭世辉，王育娜，夏信明，贺 俐，林国华，何水林++ (福建农林大学生命科学学院，福州 350002) 摘要：白藜芦醇是具有抗病及多种保健功能的芪类次生代谢物，胡萝卜(Daucus carota vat．sativa)则是富含13胡萝卜素等多 种营养成分的重要蔬菜。研究着眼于创建具有白藜芦醇保健功能的胡萝卜新种质资源，在对葡萄(Was vinifera L．)白藜芦醇合 成酶(resveratrol synthase，Rs3基因在胡萝卜中高效表达的密码子优化基础上构建无潮霉素磷酸转移酶(HPT)抗性基因的转化载 体pCB-RS-hyg，应用根癌农杆菌(Agrobactefium tumefaciens)介导法将pCB和pCB．RS-hyg-共转化到胡萝卜并获得再生植株。 经过T。、T 和T 代的逐代筛选，获得2个白藜芦醇含量较高(11．34和l5．23 mg／g)的转基因胡萝卜株系。 关键词：葡萄；白藜芦醇合成酶；胡萝卜；遗传转化；表达 中图分类号：S188 文献标识码：A 文章编号：1006．1304(2007)06．0987．05 Transformation of Resveratrol Synthase(RS)Gene of Grape into Carrot and Screening of lts Transgenic Plants with High Content of Resveratral LIN Ming，CAO Lei，GUO Shi-hui，WANG Yu-na，XIA Xin-ming，HE Li，LIN Guo-hua，HE Shui-lin (CollegeofLifeSciences，FujianAgriculture andForestryUniversity,Fuzhou350002，China) ^b—l|ct Resveratrol iS a member of stilbenoids with disease resistant activity for plants and healthy value for human．Carrot (Daucus carota)is one of the most important vegetables rich in 13一carotene．To further improve the healthy quality of D．carom,the gene ofresveratrol synthase(RS)，a key branch point enzyme for the synthesis ofresveratrol，Was transformed into carrot to acquke transgenic plants with high content ofresveratro1．The gene ofresveratrol synthase Was isolated from grape(Vitis vinifera)and the codons for amino acids were optimized for their efficient expression in carrot．With the codon optimized full length cDNA，the trans- form ation vector Was constructed by inserting the cDNA into a transformation vector pCB，whose hygromycin B phosphotransferase (HPT)gene was eliminated，then the plasmids ofpCB and pCB-RS-