荧光光谱法研究全氟辛酸与牛血清白蛋白的相互作用.pdfVIP

中国环境科学 2010,30(11) ：1496~1500 China Environmental Science 荧光光谱法研究全氟辛酸与牛血清白蛋白的相互作用 * 谢显传,王晓蓉 ,张幼宽,吴颖欣,薛银刚 (污染控制与资源化研究国家重点实验室,南京大学环境学院,水科学 研究中心,江 南京 210093) 摘要：在模拟生理条件下(pH7.4),采用荧光光谱研究环境污染物全氟辛酸(PFOA)与牛血清白蛋白(BSA) 的相互作用. 结果表明,PFOA 对 5 4 4 BSA 有荧光静态猝灭作用.PFOA 与 BSA 在温度 277,298,310K 的反应结合常数分别为 1.06×10 ,7.12×10 ,5.68×10 L/mol;在 BSA 上有 1 个 PFOA 的结合位点,该位点更接近于色氨酸残基. 由反应的热力学可推断 PFOA 与 BSA 的相互作用力主要是静电引力和疏水作用力.与 PFOA 结合后,BSA 分子构象发生变化,色氨酸残基附近疏水性增强,蛋白质分子结构趋于折叠状态;而酪氨酸残基附近亲水性增强,蛋白质 分子结构趋于松散状态. 关键词：全氟辛酸(PFOA) ；牛血清白蛋白(BSA) ；荧光猝灭 中图分类号：X132 文献标识码：A 文章编号：1000-6923(2010)11-1496-05 Interaction of perfluorooctanoic acid (PFOA) with bovine serum albumin (BSA) by fluorescence spectroscopy. XIE * Xian-chuan, WANG Xiao-rong , ZHANG You-kuan, WU Ying-xin, XUE Ying-gang (State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Center for Hydrosciences Research, Nanjing University, Nanjing 210093, China). China Environmental Science, 2010,30(11)：1496~1500 Abstract ：The interaction between perfluorooctanoic acid (PFOA) and bovine serum albumin (BSA) in physiological buffer (pH7.4) was investigated by fluorescence quenching techniques. The results showed that PFOA could strongly quench the intrinsic fluorescence of BSA through a static quenching procedure. The binding constants (K) of PFOA with BSA at 277K, 298K and 310K were 1.06×105 4 4 , 7.12×10 and 5.68×10 L/mol respectively, and the binding site (n) on BSA for PFOA was 1. The thermodynamic parameters indicated that the interaction of PFOA with BSA was driven mainly by