超高效液相色谱-质谱定量测定全血中的13种苯二氮卓类安眠镇静药物.pdf

刑事技术 2016 年 第41 卷 第1 期 ·论  著· DOI ：10.16467/j.1008-3650.2016.01.009 超高效液相色谱-质谱定量测定全血中的13种苯二氮卓类 安眠镇静药物 1 1 1 1 2 3 王朝虹 ，张 琳 ，赵 蒙 ，刘 勇 ，褚建新 ，蒋文慧 （1. 最高人民检察院检察技术信息研究中心，北京 100040 ；2. 浙江省检察院，杭州 310012 ；3. 杭州市检察院， 杭州 310000） Simultaneous Quantification of Thirteen Benzodiazepines and Metabolites in Whole Blood by UPLC-MS/MS WANG Zhaohong1, ZHANG Lin1, ZHAO Meng1, LIU Yong1, CHU Jianxin2, JIANG Wenhui2 ( 1.Procuratorial Technical Information Center of the Supreme People’s Procuratorate, Beijing 100040, China; 2.Zhejiang Provincial Procuratorate, Hangzhou 310012, China; 3. Hangzhou Procuratorate, Hangzhou 310000, China ) ABSTRACT: A rapid and sensitive method has been developed for simultaneous determination of 13 benzodiazepines and metabolites including diazepam, nitrazepam, bromazepam, flurazepam, clonazepam, flunitrazepam, lorazepam, oxazepam, prazepam, temazepam, 7-amino-nitragepam, 7-aminoflunitrazepam and chlordiazepam in whole blood samples by ultra- performance liquid chromatography coupled with mass spectrometry (UPLC-MS/MS). The separations were carried out on an ACQULTY UPLC BEH C18 column (2.1 mm ×100 mm, 1.7 μm) at 35°C and with a constant flow rate of 0.5 mL/min and the gradient-elution consisting of acetonitrile and 5 mmoL ammonium hydrogen carbonate buffer. The MS parameters were set as capillary voltage (3 kV), desolvation gas temperature (550°C), desolvation gas flow (800 L/h), cone gas flow (150 L/h) and collision gas flow (0.15 mL/min). OstraTM 96-well plate was used to extract the samples. 100 μL of human whole blood containing target drugs and D5-diazepam as internal standard was transferred into the OstraTM 96-well plate, followed by 400 μL of