多囊卵巢综合征动物模型构建的比较研究.pdfVIP

重庆医科大学学报2008年第33卷第4期 (Journal of Chongqing Medical University 2008．Vo1．33 No．4) 一 41 9一 论 簧 文章编号：0253—3626(2008)04—0419—04 多囊卵巢综合征动物模型构建的比较研究 田 秀。血 彦 (重庆医科大学附属第一医院辅助生殖中心，重庆 400016) 【摘 要】目的：比较两种多囊卵巢综合 (Polycystic ovary syndrome，PCOS)大鼠模型的构建方法。方法：模型组i 10只大鼠使 用脱氢表雄酮(DHEA)+胰岛素(INS)造模；模型组ii 10只大鼠使用绒毛膜促性腺激素(HCG)+胰岛素(INS)造模，主要观察 指标为阴道脱落上皮细胞涂片，卵巢组织学改变，血清性激素水平，空腹胰岛素值及空腹血糖值并计算HOMA指数 (HOMA—IR)。结果：组i、组ii中失去规律动情周期的大鼠的比例分别为90％、100％；两组大鼠卵巢重量显著高于相应对照 组；两组大鼠卵巢均可见多个扩张的囊泡，平均囊泡直径组 i(0．048±0．012)显著小于组ii(0．069±0．036)，黄体计数组 i (0．10±0．32)显著少于组ii(0．70±0．82)；两组与相应对照组大鼠相比较血清T水平、LH／FSH比值均有显著性升高，Ez升高不 明显(P0．05)；两模型组胰岛素抵抗倾向显著( ，0．05)，HOMA—IR模型组i(8．89±0．86)显著高于模型组ii(6．86±0．48)。结 论：脱氢表雄酮+胰岛素的造模方法在卵巢形态学改变及胰岛素抵抗程度等指标上较符合目前PCOS的特征。 【关键词】多囊卵巢综合征；动物模型；雄激素 【中国图书分类法分类号】R7l1．5 【文献标识码】A 【收稿日期】2007—10—03 Comparison of polycystic ovary syndrome models in rats N Jun．et al ( e Center of Assisted Reproduction，the First Affiliated Hospital，Chongqing Medical University) 【Abstract】Objective：Tow kinds of Polycystic Ovary Syndrome models in rats were compared．Methods：In the model i，ten female rats were given DHEA+INS．In the model ii，ten female rats were given HCG+INS．Then the ovulation，morphology of ovary．blood serum levels of sexual hormones．fasting plasma glucose(FPG)，fasting insulin(FINS)in the two models were assyed．Results：There were 90 percent of rats losting regular estrous cycle in the model i and 100 percent of rats in the model ii．The ovary weight of rats in both two models was heavier than that of controls．Rats of both two models showed polycystic ovary—like syndrome manifestation．The average saccular ovulas in model i(0．048±0．01 2)Was smaller than those in model ii(0．069±0．036)，The number of corpora lutea in model i(0．10±0．32)was smaller than that in model ii(0．70±0．82)． Serum levels of T，LH／FSH inc