NMR Protein-Ligand Interaction Studies under Non-Homogeneous Conditions for Biomaterial Generation: A Model for Artificial Lectin-Carbohydrate Recognition.pdf
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JournalofMaterialsScienceandEngineeringB2(12)(2012)618-625
蔫 l箱 H § 堪
NMRProtein--LigandInteractionStudiesunder
Non--HomogeneousConditionsforBiomaterial
Generation:A ModelforArtificialLectin——Carbohydrate
Recognition
CristinaAiroldi,SilviaMerlo,ErikaSironi1
, FrancescoNicotra andJesfisJim6nez—Barbero,
DepartmentofBiotechnologyandBiosciences,UniversityofMilano—Bicoeca,20126,Milan,Italy
2 CentrodeInvestigacionesBiol6gicas,CSIC,28040Madrid,Spain
Received:August29,2012/Accepted:September16,2012/Published:December25,2012
Abstract:Smartbiomaterialsfortissueregenerationneedtoincorporatemoleculesabletointeractwithspecificcellularadhesionor
morphogenicproteinsoftheextracellularmatrix(ECM).NMRbindingstudiesallowobtainingstrncturalinofrmationessentialforthe
comprehensionofbiologicalprocessesand,nowadays,high—resolutionmagic—angle—spinning(HR-MAS)NMR spectroscopyisa
well—establishedtoo1forthestudvofheterogeneoussystems.Herewepresentthegenerationofamode1.system usedtoexplorethe
possibilitytorevealjnteractionsbetweentwomolecularentities.oneofwhichlinkedtoasolidsupport.tomimicabioactivespecies
immobilizedonabiomaterialsurface.ispresented.ThecarbohydraterecognitionprocessesthattakeplaceintheECM haveapivotal
roleinpromotingcelladhesionanddifferentiationand,thus,tissueregeneration.Hence,weprepraedapseudo-receptor,thatmimics
thelectinbindingsitewaspreparedandwecharacterizeitsinteractionwithapanelofdifferentmonosaccharideswascharacterized.The
resultsobtainedsupportthetheoreticalmodeiaccordingtowhichlectinsbindcarbohydratesexploitingtheCH.7【interactionsoccurring
intheiractivesite.MoreovertheNMR experimentalapproachheredescribedcanbegenetallyappliedwhentheinteractingspeciesdo
nothavethe same solubility propertiesin physiologicalconditionsand,in particular,canbe exploited ofrtheanalysis and
characterizat
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