RABL-regulated pathways： a new tale in sperm function.pdf

AsianJournalofAndrolo~／(2013)15．87—88 ◎2013AJA，SIMM＆SJTU．Allrightsreserved1008．682)(／13$3200 WWW．nature．com／aja RESEARCHHIGHLlGHT RABL--regulatedpathways：anewtaleinsperm function ChariesM Allan AsianJournalofAndrology(2013)15，87—88；doi：10．1038／aja．2012．137；Publishedonline：10December2012 M mamomtilaleiflanagesplleurmmthoapvreovaidspeetchiaeluizneid—sperm motility．Femalemicecarrying the phenotpy esuggestedthatconservedRABL— R 扫2／ mutation remained fertile．Theoli— mediated actions extend to mammalian directionalpropulsionrequiredtoachieve goasthenospermia observed in infertile motileflagella．Theassemblyand function fertilisation ofthe egg．Defectiveflagella Rabl otMotmalessuggestedthattheRab21 ofmotileflagellarequiresbidirectionalintra— developmentand／orfunction isasignifi— geneencodesafactoressentialforthefunc— flagellatransport(IFT)oflargeproteincom— cantcauseofreducedsperm motliity(asth— tionaldevelopmentandmotility ofmouse plexes along a centralmicrotubule—based enospermia)andmaleinfertility．Overthe spermatozoa． structureknown astheaxoneme．Delivery pastdecade，agrowingnumberofdistinct Loeta1．ofundthatRab12isoform 1and2 ofcargoproteinsalongtheflagellaaxoneme proteinswithcrucialrolesinsperm flagella transcriptswereenrichedinmalegerm cells， involvescomponentsofIFT complexA or havebeen identified by genetic loss．of- anddetectedinmanyothertissues，including complexB machinery．In Chlamydomonas, functionapproachesinmice，inwhichthe theovaryandthosecontainingmotilefla— targetedRNA interferenceshowedthatthe deliberately end"neered or spontaneous gella／cilia(interchangeableterms)，suchas Rab—likeproteinRABL4fIFT27)formspart mutation ofin