醋制草乌不同提取物的傅里叶红外光谱法鉴定 - 国际中医中药杂志.pdf

国际中医中药杂志2013年1月第35卷第1期 Int J Trad Chin Med, January 2013, Vol. 35, No.1 ·43 · ·方药研究 · 醋制草乌不同提取物的傅里叶 红外光谱法鉴定 于琪 杜红 伊辛 武亚楠 翟文文 侯跃飞 孙素琴 王晶娟 【摘要】 目的 通过对醋制草乌及其不同提取物的红外谱图对照分析，观测药材化学成分的整体 变化规律。方法 采用傅里叶红外光谱法（FTIR ），测定供试品的红外光谱及二阶导数谱并进行分析。结 果 1713 cm－1 的C ＝O 吸收峰作为草乌中双酯类生物碱的特征峰，在不同种醋泡未煮的样品中依然能见 到该吸收峰，而玫瑰醋和陈醋煮过的样品中该吸收峰发生位移；水煎煮提取物 1713 cm－1 的C ＝O 吸收峰 不太明显，乙醚超声提取物 1712 cm－1 C ＝O 吸收峰强度增强。结论 红外光谱鉴定法可用于不同醋制草 乌的鉴别；草乌炮制过程需要先泡后煮，否则草乌水解不完全；乙醚超声提取物的红外光谱可更好的监 测草乌样品的炮制程度。 【关键词】 醋制草乌；提取物；傅里叶红外光谱法；二阶红外光谱 FTIR identification of different extract from vinegar prepared kusnezoff monkshood root YU Qi, DU Hong, YI Xin, WU Ya-nan, ZHAI Wen-wen, HOU Yue-fei, SUN Su-qin, WANG Jing-juan*. *School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China Corresponding author：WANG Jing-juan, Email：jingjuanw@163.com 【Abstract 】 Objective To observe the overall variation of the chemical composition through control analysis of vinegar Kusnezoffii extract by infrared spectrum. Methods Determine the infrared spectra (FT-IR) and secondary derivative IR spectra by FTIR. Results As 1713 cm－1 C ＝O absorption peak was set as characteristic peak for aconitum diesters Alkaloids, then this peak could be seen in the samples of uncooked while soaked in different species vinegar; the absorption peak displacement in rose vinegar and vinegar boiled samples; 1713 cm－1 C ＝O absorption peak was less obvious in boiling water extract, 1712 cm－1 C ＝O absorption peak was intensified in ether ultrasonic extract. Conclusion This identification method can be used for identifying different vinegar Kusnezoffii. The processing of Kusnezoff Monkshood root should following the steps of being soaked at first and then cooked; otherwise it will not b