天山雪莲悬浮细胞培养物中紫丁香苷的分离及含量测定 - 药学学报.pdf

436 药学学报 Acta Pharmaceutica Sinica 2009, 44 (4) : 436439 天山雪莲悬浮细胞培养物中紫丁香苷的分离及含量测定 1, 2 1 2* 1* 陈日道 , 邹建华 , 贾景明 , 戴均贵 (1. 中国医学科学院、北京协和医学院药物研究所, 北京 100050; 2. 沈阳药科大学中药学院, 辽宁 沈阳 110016) 摘要: 紫丁香苷为天山雪莲的主要活性成分之一，本研究运用溶剂提取方法，结合各种色谱手段对天山雪莲 组织细胞培养物中的紫丁香苷进行分离纯化，并根据现代谱学方法对其结构进行鉴定。同时采用高效液相色谱 法建立了天山雪莲组织细胞培养物中紫丁香苷的含量测定方法，该方法简 ，精密度、重现性良好，结果准确 可靠。这些研究为通过天山雪莲细胞悬浮培养大规模生产活性成分紫丁香苷奠定了基础。 关键词: 天山雪莲; 紫丁香苷; 细胞悬浮培养; RP-HPLC 中图分类号: R284.1; R284.2 文献标识码:A 文章编号: 0513-4870 (2009) 04-0436-04 Isolation and analysis of syringin in cell suspension cultures of Saussurea involucrata 1, 2 1 2* 1* CHEN Ri-dao , ZOU Jian-hua , JIA Jing-ming , DAI Jun-gui (1. Institute of Materia M edica, Chinese Academy of Medical Sciences and Peking Union M edical College, Beij ing 100050, China; 2. School of Traditional Chinese M edicine, Sheny ang Pharmaceutical University, Sheny ang 110016, China) Abstract: Syringin is one of the main bioactive ingredients in Saussurea involucrata. In this study, various chromatographic techniques were employed to isolate and purify syringin in the polar extraction of cell suspen- sion cultures of S. involucrata. The structure of syringin was characterized by the analysis of spectroscopic data. A quantitative analytical method for the content of syringin in cultures of S. involucrata was established with RP-HPLC. The method is convenient, accurate and reliable. All this results provided a basis for further studies on application of cell suspension cultures of S. involucrata for l