Biopolymer production by a Klebsiella oxytoca isolate using whey as fermentation substrate:(克雷伯氏菌生物聚合物生产的oxytoca隔离使用乳清作为发酵基质).pdfVIP
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Biotechnology Letters, Vol 19, No 2, February 1997, pp. 127–130
11
Biopolymer production by a
Klebsiella oxytoca isolate using whey
as fermentation substrate
A.M. Dlamini and P.S. Peiris*
11
School of Applied and Environmental Science, University of Western Sydney – Hawkesbury, Bourke Street,
Richmond, NSW 2753, Australia.
A strain of Klebsiella oxytoca was isolated from milk capable of completely utilising the lactose (3.5%, w/v) in whey
and producing biopolymer. In a broth containing 5% (w/v) lactose, 6.1 g/l of extracellular biopolymer was produced
in 72 h by the isolate. At a shear rate of 2 s–1 , broth viscosities of greater than 400 cP were obtained in lactose rich
(4%, w/v) media containing 0.2% (w/v) nitrogen concentration.
24 pts min base to base from Key words to line 1 of text
11
Introduction Organisms were screened for their ability to produce
Whey contains 4 to 5% (w/v) lactose, 0.8 to 1.0% biopolymers based on colony morphology (mucoid and
(w/v) proteins and trace amounts of minerals and vita- ropy), capsule production (negative stains – indian ink
mins (Horton 1993). It has a very strong polluting method and maneval staining technique, Corstvet et al.,
capacity, with a biological oxygen demand (BOD) 1982), and ability to increase the viscosity of enriched
of 40,000 to 45,000 mg/l (Kemp and Quickenden whey broths (whey containing 0.1% w/v yeast extract,
1989). Value-added products from whey have attracted
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