高压真气灭菌锅（High pressure gas sterilization pan）.docVIP

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高压真气灭菌锅（High pressure gas sterilization pan）.doc

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高压真气灭菌锅（High pressure gas sterilization pan）

高压真气灭菌锅（High pressure gas sterilization pan） Methods and precautions for the use of high pressure steam sterilizer Number of clicks: 854 release time: 2009-9-5 21:43:34 1. Use method (1) add a proper amount of water to the outer pot, put the sterilized items into the inner pot, cover the lid and twist the screw with symmetry. (2) make the pot produces steam heating, when the gauge pressure of 33.78 kPa, open the discharge valve, the cold air discharge, gauge pressure drop at this time, when the pointer down to zero, the exhaust valve closed. (3) continue to heat, steam pot, gauge pressure rise again, when the pressure in the boiler is increased to the required pressure, the fire, according to the features of sterilization items, make steam pressure needed for maintaining a certain time, then put the sterilizer or fire power, make its natural cold again after open the discharge valve slowly to remove residual air, and then you can open the fetch. 2. Precautions: (1) the items to be sterilized should not be placed too tight. (2) the cold air must be eliminated sufficiently, otherwise the temperature in the pot will not reach the specified temperature, which will affect the sterilization effect. (3) when the sterilization is completed, do not release the gas decompression, otherwise the liquid in the bottle will boil violently, flush the cork and spill out and even cause the container to burst. The pressure in the sterilizer should be reduced to equal to the atmospheric pressure before opening. (4) in a tube or bottle with a culture medium, paper or kraft paper should be covered in case of condensation. (5) in order to ensure the sterilization effect, should be regularly check the sterilization effect, the commonly used method is to sulfur powder (melting point is 115 ℃) or benzoic acid (melting point is 120 ℃) in vitro, then sterilization experiment was carried out. If the above material melts, the temperature in the high-pressure steam sterilizer is required, and the