异甘草酸镁对大鼠肝星状细胞增殖及活力影响.docVIP

异甘草酸镁对大鼠肝星状细胞增殖及活力影响【摘要】 目的 观察异甘草酸镁对大鼠肝星状细胞(HSC)增殖和活力的影响。方法 用含不同浓度异甘草酸镁的培养基(0、0.01、0.1、1.0、10.0 mg/ml)培养HSC,通过MTT法和LDH法检测异甘草酸镁对HSC增殖和活力的影响,并同时观察其形态变化。结果 MTT法测得与空白对照组(0 mg/ml)比较,培养基所含的异甘草酸镁浓度为1.0 mg/ml时,对HSC具有明显的抑制增殖作用;10.0 mg/ml时,其抑制增殖作用达到最高峰。LDH法测得各浓度组的异甘草酸镁给药组细胞上清液中LDH活力无明显差异。结论 异甘草酸镁在一定剂量范围内对HSC具有明显的抑制增殖作用,但对细胞上清液LDH活力无明显的影响。 【关键词】 异甘草酸镁;HSC-T6细胞株;增殖;活力 Effects of magnesium isoglycyrrhizinate on the proliferation and activityof rat hepatic stellate cell LONG Su-jun,PENG Zhong-tian,LIU Ying-xia,et al.Department of Infectious Diseases,the First affiliated Hospital of Nanhua University,Hunan,Hengyang 421001,China 【Abstract】 Objective To investigate the effects of magnesium isoglycyrrhizinate on the proliferation and activityof rat hepatic stellate cell.Methods HSC were incubated with various concentrations of magnesium isoglycyrrhizinate solution(0 mg/ml,0.01 mg/ml,0.1 mg/ml,1 mg/ml,and 10 mg/ml).With the MTT colorimetric assay and LDH,the effects of maganesium isoglycyrrhizinate on the proliferation and activityof rat hepatic stellate cell cultured were studied,and morphological changes of the HSC also observed.Results Compared with the control group(0 mg/ml group),the proliferation of HSC significantly decreased when the concentration of magnesium isoglycyrrhizinate in mediumreached 1.0 mg/ml.The restrain proliferation of HSC presented its peak when the magnesium isoglycyrrhizinate concentration was 10.0 mg/ml.Different concentrations of Magnesium isoglycyrrhizinate do not affect the vitality on HSC by LDH measurement.Conclusion Magnesium isoglycyrrhizinate could significantly restrain the proliferation of HSC within certain concentration range.But do not affect the vitality on HSC by LDH measurement. 【Key words】 Magnesium isoglycyrrhizinate; Hepatic stellate cells T6; Proliferation; Activity 作者单位:421001湖南省衡阳南华大学附属第一医院感染科(龙素军 彭忠田 刘映霞 李佳 王培);深圳市第三人民医院(刘映霞) 肝纤维化是多种慢性肝病的共同病理基础。肝纤维化发展的各个时期,肝星状细胞(hepatic stellate cell,HSC)的活化和细胞外基质合成与降解的失衡是重要