我院临床标本中分离革兰阴性杆菌菌种分布及耐药性研究.docVIP

我院临床标本中分离革兰阴性杆菌菌种分布及耐药性研究【摘要】 目的了解湖南省湘乡市中医院革兰阴性(G-)杆菌标本来源、菌种分布及耐药情况。 方法 收集2006年1月至2008年12月间临床各科送检标本做微生物病原学检测和药物敏感试验。 结果 共分离1190株G-杆菌,其中大肠埃希菌459株(38.57%)、铜绿假单胞菌214株(17.98%)、不动杆菌168株(14.12%)、肺炎克雷伯菌114株(9.58%)、阴沟肠杆菌104株(8.74%)、其他G-杆菌131株(11.01%)。其标本主要来源于痰液(54.78%)。所分离菌种对亚胺培南、阿米卡星、哌拉西林/他唑巴坦、头孢他啶、头孢噻肟、左氟沙星耐药率较低,依次为4.08%、20.28%、24.45%、33.65%、38.01%、40.19%。对氨苄西林耐药率最高,达94.31%。超广谱β-内酰胺酶检出率呈逐年上升趋势。 结论 G-杆菌分离自呼吸道,菌种分布以大肠埃希菌和铜绿假单胞菌为多,耐药菌株有逐年上升的趋势,需引起重视。 【关键词】 革兰阴性菌;抗药性,微生物;超广谱β-内酰胺酶;抗感染药 The analysis of distribution and resistance of gram -negative bacilli in the separation of our hospital’s clinical specimens ZOU Shou-hua Clinical Laboratory, the Chinese Medicine Hospital of Hunan province Xiangxiang City Hunan, Xiangxiang 411400,China 【Abstract】 Objective To study the source,distribution and antimicrobial resistance of gram-negative bacilli isolated in our hospital. Methods Pathogenic detection and antimicrobial sensitivity tests were performed for samples collected from clinical department from January 2006 to December 2008.Results 1190 gram-negative bacilli strains were isolated,459(38.57%)of which were Escherichia coli, 168(14.12%) were Acinetobacter,214(17.98%)were Pseudomonas aeruginosa,114(9.58%)wereKlebsiella pneumoniae,104(8.74%)were Escherichia cloacae,131(11.01%)were the rest of Gram-negative bacilli.,most strains(54.78%) were from sputum samples.The antimicrobial resistant rate of all the isolated bacterias to Imipenem,amikacin,pipracillin/Tazobactam,ceffazidime,Cefotaxima,levofloxacin was low, which was as follows:4.08%, 20.28%, 24.45%,33.65%, 38.01% and 40.19% respectively.The resistant rate to ampicillin was 94.31%.The isolation rate ofESBLs strains had an increasing tendence. Conclusion Gram-negative bacilli are mainly isolated from respiratory tract. The main isolated bacteria are Escherichia coli and Pseudomonas aeruginosa,the resistant bacteria strains are increasing every year. 【Key words】 Gram-negative bacilli ;Drug resistance; Microbial;Ext