拳参乙醇提取物的免疫调节作用 - 中国现代应用药学.pdf

拳参乙醇提取物的免疫调节作用 李珂珂 a b ，栾希英 (滨州医学院，a.药学院；b.基础学院，山东 烟台 264003) 摘要：目的 研究拳参乙醇提取物(BRE)的免疫调节作用。方法 在小鼠体外实验中，通过比色分析法检测小鼠巨噬细胞 系 RAW264.7 细胞吞噬中性红的能力。在小鼠体内实验中，通过测定胸腺、脾脏重量并计算脏器指数；MTT 法检测 T 淋 巴细胞增殖和 NK 细胞活性；鸡红细胞免疫后测定小鼠血清溶血素抗体水平；ELISA 法测定血清中 IL-2 含量。结果 BRE 能够显著增强 RAW264.7 细胞的吞噬能力，增加正常小鼠胸腺和脾脏重量，促进 T 淋巴细胞增殖，增强 NK 细胞的细胞毒 作用，上调血清溶血素水平及血清 IL-2 水平。结论 BRE 具有一定的免疫调节作用。 关键词：拳参；乙醇提取物；免疫调节 中图分类号：R965.1 ；R285.5 文献标志码：A 文章编号：1007-7693(2011)01-0021-05 Immunoregulatory Function of Bistortae Rhizoma Ethanol Extract a b LI Keke , LUAN Xiying (Binzhou Medical University, a.College of Pharmacy, b.College of Basic Sciences, Yantai 264003, China) ABSTRACT: OBJECTIVE To study immunoregulatory function of Bistortae Rhizoma ethanol extract (BRE). METHODS The percentage of phagocytosis of neutral red by RAW264.7 cell line from mouse macrophages was detected by colorimetric assay, in vitro. In vivo normal mice, weigh the immunological organs, MTT method was employed to investigate T lymphocyte transformation efficiency and the cytotoxicity of NK cells; measurement of the level of serum hemolysin antibody in mice immunized by chicken red blood cells and the content of IL-2 in serum with ELISA. RESULTS BRE can improve phagocytosing function of RAW264.7 macrophage cell line, increase spleen index and thymus index, promote proliferation of T lymphocyte, increase the cytotoxicity of NK cells, raise the level of serum hemolysin antibody and the content of IL-2 in normal mice. CONCLUSION BRE possesses a potential immunoregulatory function. KEY WORDS: Bistortae Rhizoma; ethanol extract; immunoregulatory 拳参又名紫参、草河车，蓼科植物拳参 毒、消肿止血的功效，用于赤痢、热泻、肺热、 (Polygonum bistorta L.) 的干燥根茎，具有清热解 咳嗽、痈肿、口舌生疮、吐血、痔疮出血、毒蛇 基金项目：滨州医学院科技计划项目(BY2007KJ09) 作者简介：李珂珂，女，硕士，讲师