中药复方对糖尿病合并脂肪肝大鼠氧化应激影响.docVIP

中药复方对糖尿病合并脂肪肝大鼠氧化应激影响[摘要] 目的：观察糖肝康对实验性2型糖尿病合并脂肪肝大鼠氧化应激的影响。方法：采用SD大鼠链脲佐菌素（STZ）25 mg/kg腹腔注射建立2型糖尿病模型，尾静脉血糖证实造模大鼠血糖异常后，改为高脂饲料喂养，制造45只2型糖尿病合并脂肪肝大鼠模型，将其随机分为5组，进行药物灌胃。糖肝康组给予糖肝康颗粒用蒸馏水溶解为适当浓度悬浊液（3.0 g/kg），二甲双胍组给予二甲双胍粉末蒸馏水悬浊液（0.08%），血脂康组予血脂康胶囊蒸馏水悬浊液（2.4 g/kg），每天每只大鼠灌胃4 ml，正常组和模型组给予等体积生理盐水灌胃。7周后，检测实验性2型糖尿病合并脂肪肝大鼠空腹血糖、胰岛素、血脂及谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）水平。结果：糖肝康能显著降低实验性2型糖尿病合并脂肪肝大鼠空腹血糖、血脂水平，同时能提高抗氧化相关酶的活性，与模型组比较差异有统计学意义（P＜0.05）。结论：糖肝康对2型糖尿病合并脂肪肝大鼠有降低血糖、调节血脂、抗氧化的作用。 [关键词] 2型糖尿病；脂肪肝；氧化应激 [中图分类号] R587.2 [文献标识码] A [文章编号] 1673-7210（2011）12（b）-026-03 Effects of traditional Chinese prescription on oxidative stress of rats with type 2 diabetic and fatty liver XU Hongmei Department of Endocrinology, Tai’an Hospital of Traditional Chinese Medicine, Shandong Province, Tai’an 271000, China [Abstract] Objective: To observe the effect of Tanggankang on oxidative stress of rats with type 2 diabetic and fatty liver. Methods: SD rats were given STZ 25 mg/kg of intraperitoneal injection to develop the model of type 2 diabetes, the glucose of tail vein was confirmed the blood sugar of the rats was abnormal, and then the rats were given fat forage. 45 rats with type 2 diabetic and fatty liver were made and randomly divided into 5 groups and fed with drugs. The group of Tanggankang was given Tanggankang particles, which were dissolved by distilled water into proper concentration of turbid liquid (3.0 g/kg), the group of Metformin was given turbid liquid of distilled water of Metformin powder (0.08%), the group of Xuezhikang was given turbid liquid of distilled water of Xuezhikang capsules, the group of normal and model was given the same volume of normal saline. After 7 weeks of drug intervention, the changes of glucose, insulin, lipid metabolism, and the plasma concentration of GSH-Px, SOD were observed. Results: Compared with the model group, Tanggankang could obviously decrease the level of the fasting blood glucose, serum lipid and improve the activities of SOD, GSH-Px, the