体外筛选川芎治疗冠心病有效成分提取纯化工艺探究.docVIP

体外筛选川芎治疗冠心病有效成分提取纯化工艺探究[摘要] 目的：通过体外筛选方法，优选川芎治疗冠心病的有效成分的提取纯化工艺。方法：培养原代乳鼠心肌细胞，利用连二亚硫酸钠（Na2S2O4）缺氧1 h复氧1 h，损伤心肌细胞制备心肌细胞缺氧-复氧损伤模型来模拟体内缺血再灌注损伤，通过体外筛选方法，优选出川芎治疗冠心病的有效成分的最佳提取和纯化工艺。结果：优选出川芎的最佳提取工艺为使用6倍量的水，提取2次，每次1 h；其纯化工艺为使用15 ml HPD-300树脂，取9 BV的0.5 g/ml的川芎提取液上样，以5 BV的50%醇洗脱。结论：通过体外筛选的提取纯化工艺可信度高，为开发研究心脑血管疾病的药物提供了依据。 [关键词] 川芎；提取；纯化；原代乳鼠心肌细胞；冠心病；连二亚硫酸钠 [中图分类号] R284.2 [文献标识码] A [文章编号] 1673-7210（2011）12（b）-081-04 The research of extraction and purification process of active ingredients of Chuanxiong which could treat coronary heart disease through screening methods in vitro REN Cong, MENG Xiansheng, BAO Yongrui College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Liaoning Province, Dalian 116600, China [Abstract] Objective: To select the best extraction and purification process of active ingredients of Chuanxiong which could treat the coronary heart disease through screening methods in vitro. Methods: Primary cultured neonatal rat cardiomyocytes and sodium hydrosulfite (Na2S2O4) were used for hypoxia 1 h and reoxygenation 1 h to form the cardiomyocyte hypoxia-reoxygenation injury model to simulated in vivo ischemia-reperfusion injury. Screening methods in vitro were used to select the best extraction and purification process of active ingredients of Chuanxiong which could treat coronary heart disease. Results: The best extraction process selected was that, Chuanxiong was extracted for 2 times, 1 h for each time with 6 times the amount of water; while the purification process was that, 15 ml HPD-300 resin was used, 9 BV of 0.5 g/ml of the Chuanxiong extract and 5 BV of 50% ethanol was applied to elute. Conclusions: The technology of extraction and purification through screening methods in vitro has high reliability, and provides a basis for the development of new drug that treat cardiovascular disease. [Key words] Chuanxiong; Extraction; Purification; Primary neonatal rat cardiomyocytes; Coronary heart disease; Sodium hydrosulfite 川芎是