Effects of Citrus sinensis (L.) Osbeck epicarp essential oil on growth英文教材.pdf

Effects of Citrus sinensis (L.) Osbeck epicarp essential oil on growth英文教材.pdf

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ARTICLE IN PRESS Microbiological Research 163 (2008) 337—344 www.elsevier.de/micres Effects of Citrus sinensis (L.) Osbeck epicarp essential oil on growth and morphogenesis of Aspergillus niger (L.) Van Tieghem Neeta Sharma, Abhishek Tripathi Mycology and Plant Pathology Division, Department of Botany, University of Lucknow, Lucknow 226007, India Accepted 15 June 2006 KEYWORDS Summary Aspergillus niger ; Essential oils from different plant parts are known for their antimicrobial activity but Citrus sinensis; the antifungal effects of essential oil from Citrus sinensis (L.) Osbeck epicarp on Essential oil; growth and morphogenesis of Aspergillus niger has not been observed so far. The GC/MS analysis; mycelial growth was inhibited at 2.5 and 3.0 mg/ml of oil in Potato Dextrose Broth Scanning electron and Agar medium, respectively. These concentrations were fungicidal under the test microscopy conditions. The fungitoxicity of oil did not change even at exposure to 100 1C and autoclaving. The main changes observed under light and scanning electron microscopy after oil treatment were loss of cytoplasm in fungal hyphae, and budding of hyphal tip. The hyphal wall and its diameter became markedly thinner, distorted and resulted in cell wall disruption. The flattened and empty hyphal tips bifurcated into bud lik

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