Single-Cell Phenotyping within Transparent Intact Tissue through Whole-Body Clearing英文教材.pdfVIP

Resource Single-Cell Phenotyping within Transparent Intact Tissue through Whole-Body Clearing Bin Yang,1 Jennifer B. Treweek,1 Rajan P. Kulkarni,1,2 Benjamin E. Deverman,1 Chun-Kan Chen,1 Eric Lubeck,1 Sheel Shah,1 Long Cai,3 and Viviana Gradinaru1,* 1Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA 2Division of Dermatology, Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA 3Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA *Correspondence: viviana@ /10.1016/j.cell.2014.07.017 SUMMARY of more recent tissue-clearing methods and modern micro- scopy techniques. While separate tissue-clearing protocols Understanding the structure-function relationships have strengths in an application-specific context, none is at cellular, circuit, and organ-wide scale requires able to fully surmount the most common obstacles: uncon- 3D anatomical and phenotypical maps, currently un- firmed generalizability across organs other than the brain or available for many organs across species. At the root embryo, difficulties in execution, and incompatibility with of this knowledge gap is the absence of a method endogenous fluorescence and/or post hoc immunohistochem- istry (Table S1). Thus motivation to improve tissue-clearing that enables whole-organ imaging. Herein, we pre- protocols is sustained around three main objectives: (1) e