低渗造影剂对老年糖尿病大鼠肾毒性探究.docVIP

低渗造影剂对老年糖尿病大鼠肾毒性探究【摘要】 目的 观察低渗造影剂欧乃派克350对老年糖尿病大鼠肾功能的影响。方法 建立老年糖尿病大鼠模型，观察尾静脉注射欧乃派克350(10 ml/kg)后，大鼠血肌酐、尿素氮以及肾组织匀浆一氧化氮(NO)、谷胱甘肽(GSH)的含量。结果 老年糖尿病大鼠血肌酐、尿素氮与正常对照组比较均升高(P0.05)，肾组织匀浆一氧化氮、谷胱甘肽与正常对照组比较均下降(P0.01，P0.05)。注射造影剂后，血肌酐与正常对照组比较明显升高(P0.01)，肾组织匀浆一氧化氮与糖尿病对照组比较下降(P0.05)。结论 低渗造影剂可以引起老年糖尿病大鼠肾损伤，可能与糖尿病大鼠NO合成或消耗失调，进一步激活NO信号通路有关。 【关键词】 老年大鼠; 糖尿病; 造影剂肾病; 一氧化氮 The effects of nephrotoxicity of iohexol in male aging rats with diabetes WANG Ruiqiang, WANG Yi, LI Tao, et al. Departmemt of Gerontology, The First Affiliated Hospital of Zhengzhou University, Open Laboratory of Unode Science of Clinical Medicine of Henan Province, Zhengzhou 450052, China 【Abstract】 Objective To investigate the effects of nephrotoxicity of iohexol in male aging rats with diabetes.Methods 40 male SD rats were included in the study. 10 rats were divided into normal control group(SD group，n=10)，the other 30 rats were induced by intraperitoneal administration of one dosage of streptotocin.At the end of the eighth weeks，the diabetic rats were divided into two groups at random：diabetic control group (DM group，n=13), diabetic rats injected with iohexol (10 ml/kg) (LOCM group，n=13).After having finished the injection for 48 hours，the levels of serum creatinine(Scr)，blood urea nitrogen(BUN) and blood glucose were examined under anesthetization；glutathione (GSH) and nitric oxide(NO) in renal cortex were measured.Results The levels of Scr and BUN increased in DM group compared with SD group (P0.05).Conclusion Iohexol can cause nephrotoxicity in aging diabetic rats.NO maybe play an important role in the nephrotoxicty of iohexo in diabetic rats. 【Key words】 Aging rats; Diabetes mellitus; Contrast induced nephropathy; Nitric oxide ________________________________________ 作者单位：450052 郑州大学第一附属医院老年病科/河南省高等学校临床医学重点学科开放实验室 随着现代医学影像诊断和介入治疗的快速发展，血管内造影剂的应用越来越普及，造影剂肾病(contrast induced nephropathy，CIN)的发病率也呈上升趋势。造影剂肾病是指血管内注射碘造影剂后48 h内，在排除其他病因的前提下，肾功能发生损害，血清肌酐水平升高0.5 mg/dl(44.2 μmol/L)或比基础值升高25％以上。由造影剂引起的急性肾功能衰竭是