离子色谱法测定试验动物环境空气中的氨.DOCVIP

研究报告 离子色谱法测定实验动物环境空气中的氨 钟汉怀，邱宏 （重庆市疾病预防控制中心，重庆 400042） [摘要] 目的建立一种用抑制电导离子色谱仪测定实验动物环境空气中氨的方法。方法 以稀硫酸作为吸收液采集空气中的氨，使氨在吸收液中转化为铵离子，选用抑制型电导检测器，CS12A阳离子分析柱，以甲磺酸作为淋洗液，通过测定吸收液中的铵离子的含量来计算空气中氨的浓度。结果 铵离子在0～1.00 μg/mL范围内具有良好的线性(r = 0.9991 )，方法精密度高(RSD6.38%)，最低检出浓度为0.012 mg/m3，方法的平均回收率98.8%。结论 该法操作简便，无干扰离子影响，可作为实验动物环境空气中氨的测定方法。 [关键词]氨;离子色谱法；实验动物环境空气 【中图分类号】R33【文献标识码】A【文章编号】1671-7856（201）-0000-00 Doi:10.3969/j.issn.1671-7856.2015.010.000 An ion chromatography method for detection of ammonia in the environmental air in laboratory animal facilities ZHONG Han-huai , QIU Hong (1. Chongqing Center for Disease Control and Prevention, Chongqing 400042, China) [Abstract] Objective To establish an ion chromatography (IC) method for detection of ammonia in the environmental air of laboratory animal facilities. Methods Dilute sulfuric acid was used as the absorption liquid to collect ammonia in the air. Ammonia was converted into ammonium ion in the absorption solution. With methylene sulfonic acid as the pouring liquid, the content of ammonium ion in the absorption solution was detected, and the concentration of ammonia in the air was calculated. Results The ammonium ion showed good linearity in the range of 0 to 1 μg/mL (r = 0.9994), and high precision (RSD6.38%). The lowest detected concentration was 0.012 mg/m3, and the average recovery was 98.8%. Conclusions This is a simple method, easy to operate, not interfered by other ions, and can be used to detect the ammonia in the environmental air of laboratory animal facilities. [Keywords] Ammonia; Ion chromatography (IC); Laboratory animal; Environmental air ［通讯作者］钟汉怀（1964-），男，大专，主管技师，主要从事实验动物环境及职业病危害因素检测。 实验动物几乎终身被限制在一个极其有限的环境范围内生活，这种环境成了实验动物赖以生存的条件。氨浓度是动态实验动物繁育、生产设施环境的重要指标，现行的标准方法是纳氏比色法［1］，使用剧毒化学试剂，对实验人员有较大的危害。本文建立了用离子色谱法测定实验动物环境中氨的方法，检出限比纳氏比色法有较大的提高，实验不需要剧毒化学试剂，对实验人员起到了很好的保护作用。 材料方法 .1原理动物环境空气中的氨用稀硫酸吸收，化学反应为NH3 + H+→ NH4+，形成的NH4+用离子色谱法定量，采用阳离子分析柱分离，抑制性电导检测离子色谱法检测，用NH4+的保留时间定性，峰面积定量。通过测定吸收液中的NH4+的浓度，计算空气中氨的含量。 .2仪器与试剂 仪器 FDC-1500防爆型大气