人类免疫缺陷病毒抗体确证试验假阳性1例分析.pdf

蚌埠医学院学报2014年11月第39卷第11期 1559 [文章编号] 1000鄄2200(2014)11鄄1559鄄03 ·检验医学· 人类免疫缺陷病毒抗体确证试验假阳性 1例分析 杨晓辉,温摇 兰 [摘要]目的:通过对1例人类免疫缺陷病毒(HIV)抗体确证试验结果假阳性者进行随访检测分析,探讨HIV抗体确证试验技 术在实际工作中存在的问题和解决的方法。 方法:对1例受检者进行HIV抗体筛查和确证试验,并于4 周、8周和12周随访 + 检测,后两次随访进行HIV鄄1病毒载量检测以及第四次进行CD4 T淋巴细胞检测。 结果:4次HIV抗体检测结果免疫印迹试 + 验带型均为gp160、gp120、p24;后两次病毒载量结果均为TND,第四次CD4 T淋巴细胞检测结果为885 cells/ 滋l,结合流行病 学资料,最终判定HIV抗体阴性。 结论:随着HIV抗体确证试验结果不确定样本不断增多,检测者要格外谨慎判断处于临界 状态的样本,必须结合流行病学资料分析及随访检测,才能给予准确的检测报告。 [关键词]人类免疫缺陷病毒;假阳性;确证试验 [中国图书资料分类法分类号] R512.91摇 摇 摇 [文献标志码]A Analysis of false positive conclusion in human immunodeficiency virus antibody confirmatory test:report of one case YANGXiao鄄hui,WEN Lan (Fuyang Centerfor Disease Control and Prevention,FuyangAnhui236030,China) [Abstract] Objective:To explore the problems existing in human immunodeficiency virus(HIV) antibody confirmatory testing techniques and the corresponding solutions in practical work by following up one case of false positive conclusion in HIV antibody confirmatory test.Methods:The subject underwent HIV antibody screening and confirmatory testing,and received follow鄄up testing at the4th week,8th week and 12th week.In the last two follow鄄ups,HIV鄄1viral load testing was conducted;and in the fourth follow鄄up, + ndtypes of HIV antibody detected by immunoblotting in thefour testswere all the CD4 T lymphocyteswere detected.Results:The ba + gp160,gp120 and p24;the viral load results in the last two