双波长补偿光度法的研究及对微量铁的测定.pdfVIP

第27卷第1期 分析测试学报 Vol_27 No．1 2008年1月 FENXI CESHI XUEBAO(Journal of Instrumental Analysis) 49～52 双波长补偿光度法的研究及对微量铁的测定 黄泽南 ，江南梅 ，于林亚 ，曹方娜 ，黄 芳 (1．湖南科技大学 化学化工学院，湖南 湘潭 411201；2．湘潭电化集团公司，湖南 湘潭 411131) 摘 要：提出了新的双波长法并应用于茜素s(ARS)光度法测定铁。该法能消除显色剂ARS褪色对生成物 Fe[ARS]，生色的相消干扰，测得生色法真实吸光度，消除生成物生色对有色剂ARS褪色的相消干扰，测得 褪色法真实吸光度，以其生色吸光度与褪色吸光度绝对值之和作为信号，按信号吸光度法测定。于波长A = 423 nm，A =555 nm测得信号吸光度补偿系数 为2．227，测得摩尔吸光系数为2．154×10 L·mol～· cm ～ ， 为经典法的2．135倍，线性范围为0—2 mg／L，反应摩尔比为1：3。应用该法测定了某些样品中的 铁。结果表明，该法用于存在相消干扰的光度分析及反应摩尔比测定等研究的效果优于经典光度法。 关键词：双波长补偿光度法；相消干扰；真实吸光度；茜素S；铁 中图分类号：0657．32；O614．811 文献标识码：A 文章编号：1004—4957(2008)01—0049—05 A Study on the Dual—wavelength Compensation Spectrophotometry and Its Application to Micro Ferrum Determination HUANG Ze—nan ，JIANG Nan—mei ，YU Lin—ya ，CAO Fang—na ，HUANG Fang (1．School of Chemistry and ChemicM Engineering，Hunan University of Science and Technology， Xiangtan 41 1201，China；2．Xiangtan Electro—chemical Corp．，Xiangtan 41 1 131，China) Abstract：A novel dual—wavelength compensation spectrophotometry was established and applied to the determination of micro ferrum with alzarin red S(ARS)as the chromogenic reagent．This method could determ ine the real absorbance of the chromogenic product in spectrophotometry through elimina— ring the destructive interference of discolored ARS on the resultant chromogenic product，Fe[ARS]3． It could also determine the real absorbance of the discolored product in spectrophotometry through e— liminating the destructive interference of Fe[ARS]3 on the discolored ARS．The sum of the absolute chromogenic absorbance and discolored absorbance was taken as the signal determined by the spectro— photometry．The compensation coefficient of absorbance signal was 2．227 at wavelengt