dependence of spreading and differentiation of mesenchymal stem cells on micropatterned surface area依赖传播和分化的间充质干细胞在微型图象表面区域.pdfVIP

Hindawi Publishing Corporation Journal of Nanomaterials Volume 2011, Article ID 265251, 9 pages doi:10.1155/2011/265251 Research Article Dependence of Spreading and Differentiation of Mesenchymal Stem Cells on Micropatterned Surface Area Wei Song,1, 2 Naoki Kawazoe,1, 3 and Guoping Chen1, 2, 3 1 Biomaterials Center, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan 2 Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8571, Japan 3 International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan Correspondence should be addressed to Guoping Chen, guoping.chen@nims.go.jp Received 16 October 2010; Revised 6 January 2011; Accepted 5 February 2011 Academic Editor: Yanan Du Copyright © 2011 Wei Song et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Micropatterning technology is a highly advantageous approach for directly assessing and comparing the effects of different factors on stem cell functions. In this study, poly(vinyl alcohol)- (PVA-) micropatterned polystyrene surfaces were prepared using photoreactive PVA and ultraviolet photolithography with a photomask. The micropatterned surface was suitable for single-cell array formation and long-term cell culture due to the nanometer thickness of nonadhesive PVA layer. Different degrees of cell spreading with the same cell shape were established by adjusting the sizes of circular, cell-adhesive polystyrene micropatterns. Cell spreading and differentiation of mesenchymal stem