肌力康饮对EAMG大鼠血清AchR-Ab及TGF-β1水平影响.docVIP

肌力康饮对EAMG大鼠血清AchR-Ab及TGF-β1水平影响摘 要:目的:观察中药复方肌力康饮对实验性自身免疫性重症肌无力(EAMG)大鼠血清AchR-Ab、TGF-β1水平的影响,以探讨其治疗重症肌无力的可能机制。方法:60只Lewis大鼠随机分为6组,每组10只:空白组,模型组,阳性药物组,肌力康饮高、中、低剂量组。采用AchR主动免疫的方法免疫除空白组外的各组大鼠,制备EAMG大鼠模型。确立模型成功,各组给药治疗4周后,采用ELISA法检测血清AchR-Ab、TGF-β1水平。结果:肌力康饮各组、阳性药物组能明显改善EAMG大鼠肌无力症状,临床症状评分显著降低、血清AchR-Ab水平明显下降、TGF-β1水平明显升高,与模型组比较,有显著性差异(P0.05)。结论:肌力康饮下调了EAMG大鼠过高的AchR-Ab水平,并使TGF-β1升高,从而改善了EAMG大鼠的临床症状。 关键词:肌力康饮;EAMG;TGF-β1;AchR-Ab 中图分类号:R285.5 文献标识码:A 文章编号:1673-7717(2010)01-0080-03 Effect of Ji Li Kang Drink on Eoperimental Autoimmune Myasthenia Gravis Serum AchR-Ab and TGF-β1 Levels NIU Feng?yun,CHEN Hui?juan,JIA Wei?gang,ZHAO Li?li (Heilongjiang Academy of Traditional Chinese Medicine, Harbin 150036, Heilongjiang, China) Abstract:Objective: Observe the effect of Ji Li Kang Drink on experimental autoimmune myasthenia gravis (EAMG) serum AchR-Ab, TGF-β1 levels in order to explore the possible mechanisms of treatment of myasthenia gravis.Methods: 60 Lewis rats were randomly divided into six groups of 10: blank group, model group, positive medicine group, high-dose group of Ji Li Kang drink ,medium-dose group and low-dose group. Method of active immunization with AchR was used to prepare for EAMG rats. After being established a successful model, each group were detected serum AchR-Ab, TGF-β1 levels using ELISA after 4 weeks drug treatment.Results: groups of Ji Li Kang drink and positive medicine group can significantly improve the symptoms of EAMG rats;Score of clinical symptom significantly decreased ; AchR-Ab levels in serum were significantly decreased;TGF-β1 levels were significantly elevated compared with model group.There was significant difference (P0.05). Conclusion: Ji Li Kang drink reduced AchR-Ab level , and increased TGF-β1 level of EAMG rats Thus Ji Li Kang drink improved the clinical symptoms ofEAMGrats. Key words:Ji Li Kang Decoction; EAMG;TGF-β1; AchR-Ab 重症肌无力(myasthenia gravis,MG)主要是由乙酰胆碱受体抗体介导的,补体参与、细胞免疫依赖的,针对神经肌肉接头处突触后膜上乙酰胆碱受体的自身免疫性