倏逝波光纤免疫传感器探头的修饰及表征Surface-上海有机化学研究所.PDFVIP

2008 66 Vol. 66, 2008 4 , 454458 ACTA CHIMICA SINICA No. 4, 454458 · · * ( 100084) . -LR (mi- crocystin-LR, MC-LR) , MC-LR (OVA) MC-LR-OVA, , XPS . , , MC-LR-OVA . MC-LR , , . , . ; ; ; ; -LR Surface Modification and Characterization of the Probe Based on Evanescent Wave Fiber-optic Immunosensor LONG, Feng* SHI, Han-Chang HE, Miao ZHU, An-Na SHENG, Jian-Wu (State Key Joint Laboratory of Environmental Simulation and Pollution Control, Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084) Abstract The modification of the surface of the fiber optic probe by bio-recognizing molecules is essential for detecting interest target by evanescent wave fiber optic immunosensor. In this study, microcystin-LR (MC-LR) was chosen as the example substance. Hapten-carrier conjugates MC-LR-Ovalbumin were synthe- sized by mixing MC-LR and ovalbumin (OVA) activated by glutaraldehyde, and then the conjugates were immobilized onto the silane layer on the probe with a heterobifunctional crosslinker. The probe modified was evaluated by XPS and an evanescent wave all-fiber immunosensor. The results showed that surface chemical compositions of the probe obviously changed at different modification steps, MC-LR-OVA was covalently bound with the probe surface, strong binding of antibody of MC-LR and low non-specific adsorp- tion to other antibody was monitored, and good