联合转染PDGF和c-myc反义寡核苷酸抑制移植血管狭窄研究.docVIP

联合转染PDGF和c-myc反义寡核苷酸 抑制移植血管狭窄研究 冯虎翼1，吴忠均2，朱宇2 （1重庆市第五人民医院2重庆医科大学附属第一医院普外科，重庆 400016） The study on platelet-derived growth factor and c-myc together inhibiting the stenosis of transplanted vascular FENG Hu-yi1 WU Zhong-jun2 YU Lin2 1 General Surgery of the No.5 people hospital of Chongqing 400062 2General Surgery of the First Affiliated Hospital, Chongqing medical University, Chongqing 400016 Abstract Objective: To study the effect of platelet-derived growth factor（PDGF）and c-myc antisense oligodeoxynucleotides(AODN) together on inhibiting the proliferation of vascular smooth muscle cells(VSMC) and the stenosis of transplanted vascular. Method: To select 48 male Zelanian rabbits and were randomly divided into four groups(n=12,in each group): control group, PDGF-AODN group, c-myc-AODN group, PDGF-AODN adding c-myc-AODN group. The left and right external iliac arteries(length 1.0cm) of rabbits were transplanted reciprocally. The transplanted vascular were respectively soaked in lipofiction, PDGF-AODN, c-myc-AODN and PDGF-AODN addingj c-myc-AODN solution about 20 minute, the vascular anastomotic were sutured by 8/0 suture of soaked in AODN solution. Four weeks later, the specimens were harvested for microscopy. The pathological morphology of transplanted vascular were observed under microscope(HE). The intimal thickness and area, stenosis ratio(%) of transplanted vascular were calculate and analysed statistically among group by computer system. The number of positive cells of PDGF’s mRNA in transplanted vascular wall were counted with in situ hybtidzatian histo-cytochemistry and the number of positive cells of c-myc’s protein in transplanted vascular wall were counted by S-P immunochemisty. Result: The intimal thickness and area, stenosis ratio of transplanted vascular, the number of PDGF and c-myc positive cell in PDGF-AODN addingj c-myc-AODN group were significantly lower than those in other group(P﹤0.01), and that were lower evidently than PDGF-AODN group and c-