肝纤维化血清标志物临床应用评价.docVIP

肝纤维化血清标志物临床应用评价[摘要] 目的 评价肝纤维化血清标志物透明质酸（HA）、Ⅲ型前胶原N端肽（PⅢNP）、Ⅳ型胶原（CⅣ）、层粘连蛋白（LN）对肝纤维化诊断的应用价值。 方法 以肝穿病理学组织检查为金标准，采用化学发光法肝纤维化血清标志物定量检测试剂检测70例患者样本，计算血清标志物的灵敏度、特异性和准确性。 结果 与金标准相比，HA、PⅢNP、CⅣ、LN的灵敏度分别为83.3%、58.3%、66.7%、50.0%，特异性分别为77.3%、81.8%、72.7%、81.8%，诊断肝纤维化的准确性分别为81.4%、65.7%、68.6%、60.0%。 结论 血清HA、LN、PⅢNP、CⅣ水平与肝组织纤维化有良好的相关性，能较好地反映肝病的慢性化程度和肝纤维化活动水平及程度，在肝纤维化诊断中具有重要的应用价值。 [关键词] 肝纤维化；化学发光；透明质酸；Ⅲ型前胶原；Ⅳ型胶原；层粘连蛋白 [中图分类号] R575.2 [文献标识码] B [文章编号] 1673—9701（2012）25—0075—02 Clinical evaluation of serum markers of liver fibrosis LI Xiaoxia1 LI Bin2 LI Lin1 LI Zhongxin1 FU Guangyu2 WU Xuewei1 1.Zhengzhou Autobio Diagnostics Co. Ltd. RD， Zhengzhou 450016， China； 2.National Local Joint Engineering Laboratory of Immunoassay Automation， Zhengzhou 450016，China [Abstract] Objective To evaluate serum markers Hyaluronic Acid（HA）， Amino—terminal Propeptide of Type III Collagen （PⅢNP）， Type Ⅳ Collagen（CⅣ） and Laminin（LN） of liver fibrosis， which is a practical application for the diagnosis of liver fibrosis. Methods Histopathological examination of liver puncture be defined as golden standard， Seventy patients which were determined by golden standard were detected， which adopting by chmiluminescence reagent of Quantitative detection to Serum markers of liver fibrosis. Then， Sensitivity， specificity and accuracy were calculated and analysed to serum markers. Results Compared with golden standard， the sensitivity of HA， PⅢNP， CⅣ and LN was 83.3%， 58.3%， 66.7 and 50.0%； the specificity was 77.3%， 81.8%， 72.7% and 81.8%； the accuracy was 81.4%， 65.7%， 68.6% and 60.0%， respectively. Conclusion The levels of HA， PⅢNP， CⅣ and LN in serum is well in correlation with liver fibrosis， which would well reflect the level of chronic patients and liver fibrosis. There is importantly actual application value on diagnosis of liver fibrosis. [Key words] Liver Fibrosis； Chemiluminescence； Hyaluronic acid； Amino—terminal Propeptide of Type Ⅲ Collagen； Type Ⅳ Collagen； Laminin 肝纤维化是由各种致病因子所致肝内纤维组织异常增生