spectroscopic studies on the interaction of hypocrellin a with myoglobin光谱的hypocrellin与肌红蛋白相互作用的研究.pdfVIP

Spectroscopy 21 (2007) 235–243 235 IOS Press Spectroscopic studies on the interaction of hypocrellin A with myoglobin J.H. Zhou a , X.H. Wu a , C. Yang c , X.T. Gu a , L. Zhou b , K.X. Song a , Y.Y. Feng a,∗ and J. Shen a,∗ a Analysis Testing Center, Jiangsu Engineering Research Center for Bio-medical Function Materials, Nanjing Normal University, Nanjing, 210097, P.R. China b School of Life Science, Nanjing Normal University, Nanjing, 210097, P.R. China c Department for intensive instruction, Nanjing Normal University, Nanjing, 210097, P.R. China Abstract. Experimental results of UV-visible absorption spectroscopy and fluorescence spectroscopy indicate that hypocre- llin A, which has been studied in photodynamic therapy, can interact with the surface of myoglobin through hydrophobic forces, and form a complex. Based on the Stern–Volmer equation, the quenching constants of the process can be calculated to be 4 .81 × 1012 L mol−1 s−1 (t = 25◦C) and 4.54 × 1012 L mol−1 s−1 (t = 42◦C) respectively, and the binding constant is 4 M−1 (t = 25◦C), while the binding sites is 0.94 (t = 25◦C). In addition, Electron paramagnetic resonance and 5.53 × 10 fluorescence spectroscopic analysis suggests that that the quenching mechanism of the interaction process occurs through the electron transfer between hypocrellin A and myoglobin. Keywords: Hypocrellin A, myoglobin, UV-visible absorption spectroscopy, fluorescence spectroscopy, electron paramagnetic resonance spectroscopy 1. Introduction Hypocrellin A (4,9-dihydroxyperylene-3-10-quinone, HA), one kind of natural pigment, which can be extracted from Hypocrella bambuase, a parasitic fungus of Siramudinaria, has been used as a photother- apeutic agent to cure various skin diseases. It is also an orally taken folk medicine for several centuries in China [1,2]. Recently, this nat