spectroscopy in heterogenous media and applications for bioprocess and environmental monitoring光谱学在异构媒体和应用生物处理和环境监测.pdfVIP

Vol. 4 INTERNATIONAL JOURNAL OF PHOTOENERGY 2002 Spectroscopy in heterogenous media and applications for bioprocess and environmental monitoring Frank Schael, Oliver Reich, and Sonja Engelhard Institut für Physikalische Chemie und Theoretische Chemie, Universität Potsdam, Karl-Liebknecht-Str .24-25, D–14476 Golm, Germany Abstract. Diffuse reflectance measurements and photon migration studies with near infrared (NIR) diode lasers were employed to elucidate experimental methods for determining absorption and scattering coef- ficients and species concentrations in heterogenous media. Measurements were performed at a number of wavelengths utilizing several laser sources some of which were widely tunable. In order to establish the applicability of simple photon migration models derived from radiation transport theory and to check the experimental boundary conditions of our measurements, simple light scattering solutions (such as suspen- sions of titanium dioxide, latex particles, and solutions of milk powder) containing dyes (such as nile blue, isosulfan blue) were investigated. The results obtained from diffuse-reflectance studies at different source- detector distances were in accordance with predictions from simple photon diffusion theory. Applications of reflectance measurements for monitoring of cell growth during fermentation processes and for in-situ investigations of soils are presented. 1. INTRODUCTION applications have given rise to a new class of optical near infrared (NIR) sensors with imp