病原问答题（Pathogen question）.docVIP

病原问答题（Pathogen question） 1. Comparing the biological characteristics of prokaryotic cells, the main differences between eukaryotic cells and non cell types of microorganisms? A: prokaryotic cells: a cell-free structure that lacks an independent enzyme system and can only live in live cells Prokaryotic cell type: cell structure, but low cell differentiation, nuclear membrane without nuclear membrane, nucleolus, organelle is not perfect Eukaryotic microorganisms: complete cell structure, high degree of cell differentiation, complete nuclear and organelle 2. Discuss the procedure, result and medical significance of Gram staining method Answer: steps: smear and liquid crystal violet dye, mordant, iodine solution at the beginning of 95% ethanol decolorization, carbolfuchsin staining, oil microscope Results: gram positive bacteria were dyed purple and gram negative bacteria were dyed red Significance: identifying bacteria, guiding clinical medication and understanding the pathogenicity of bacteria 3. The conditions of propagation, propagation, speed and growth law of bacteria are briefly described Answer: adequate nutrients, suitable pH, the appropriate temperature, a certain amount of gas Way: two split Speed: quick! Law: divided into slow, logarithmic growth phase, stable period, decline period 4. What are the medical implications of bacterial anabolic products? Answer: metabolites associated with the pathogenicity of bacteria and toxins, invasive enzymes, pyrogen For the identification of bacteria or type of pigment, pyrogen For treatment: antibiotics, vitamins 5. Briefly describe the significance of artificial culture of bacteria Answer: for pathogenic diagnosis and drug sensitivity test, preparation of vaccines, for bacterial research, for genetic engineering 6. Describe the physiological significance of normal flora Answer: nutrition, biological antagonism, immune, anti-cancer effect, anti-aging effect 7. Describe the pathogenic cause of conditional pathogenic bacte