质粒提取的原理（Principle of Plasmid Extraction）.doc

质粒提取的原理（Principle of Plasmid Extraction） Preparation of plasmids from Escherichia coli by alkaline lysis is a routine technique used in laboratory studies of molecular biology every day. But I have been a graduate student for more than ten years, and almost without exception, my excellent students who know everything know very little about the principle of alkaline plasmid extraction. The reason for this, I think, is probably because the molecular cloning contains experimental operations only, without a detailed exposition of the principles. This is the main reason that leads my students astray. Later, I found that in fact, the level of graduate students in all related fields in China was almost the same, and even a lot of teachers were in the same state. This has to make people feel sad. Im afraid it has something to do with our culture. Chinese advocate of reading, official concept. Often hear parents on their only child, as long as you concentrate on reading books on it. Therefore, the definition of reading is to remember what is written in the book and get high marks when taking exams...... This is the fundamental reason why modern science did not germinate in china. If Chinese culture does not change at this point, it is not really science rooted in China, it can only degenerate into eight shares of the new school . Life science is experimental science. It stresses practice. If experimental science is enough to look at books, I would like to ask the immortal who would read a book and ride a bicycle Or listen to the sports teachers explanation, you will skate? But instead of thinking, you become a craftsman A qualified life science researcher needs to improve himself in these two areas. An outstanding scientist, an artist who knows scientific principles and is good at using them. Every student who has extracted plasmids with alkaline method, I hope you can think about it after this article, so that the future of China will be promising To make sense, here are th