土壤酸杆菌门细菌生态学研究进展-生物技术通报.PDF

生物技术通报 ·综述与专论· BIOTECHNOLOGY BULLETIN ( ) 2016, 32 2 :14-20 土壤酸杆菌门细菌生态学研究进展 王光华  刘俊杰  于镇华  王新珍  金剑  刘晓冰 （中国科学院东北地理与农业生态研究所 中国科学院黑土区农业生态重点实验室，哈尔滨 150081） 摘 要 ： 酸杆菌是土壤中一类重要的细菌类群。基于 16S rRNA 基因序列分析发现，酸杆菌一般占细菌总量的20% 左右， 甚至高达50% 以上，表明酸杆菌在土壤生态过程中起到重要的作用。从植被、海拔高度、氮肥管理及二氧化碳升高对土壤酸杆菌 分布的影响，以及酸杆菌根际效应等几方面论述了土壤酸杆菌门细菌生态学研究进展；综合分析揭示出不同分类单元酸杆菌细菌 分布与环境因子间的关系；阐述了部分酸杆菌细菌的潜在生态功能。最后指出在土壤酸杆菌研究中应强化分离培养、细化分子生态、 采用宏基因组学和单细胞测序新技术的重要性。 关键词 ： 酸杆菌 ；微生物生态 ；亚群 ；16S rRNA 基因 ；高通量测序 DOI ：10.13560/j.cnki.biotech.bull.1985.2016.02.002 Research Progress of Acidobacteria Ecology in Soils - - - - - WANG Guang hua LIU Jun jie YU Zhen hua WANG Xin zhen JIN Jian LIU Xiao bing （Key Laboratory of Mollisols Agroecology ，Northeast Institute of Geography and Agroecology ，Chinese Academy of Sciences ，Harbin 150081 ） Abstract: The phylum Acidobacteria is one of the most important bacterial groups in soils. Based on analysis of 16S rRNA gene sequences, the number of Acidobacteria generally represents about 20% of total soil bacterial communities, some of them even account for more than 50%, which suggests that Acidobacteria play important roles in soil ecological process. In this paper, we reviewed the research progress of Acidobacteria ecology in soils from several aspects, such as effects of plant types, altitude, nitrogen fertilizer and CO2 enrichment on the distribution of Acidobacteria , as well as rhizosphere effects of Acidobacteria , etc. The relationships between distributions of different taxonomic level Acidobacteria and environmental factors were summarized. The potential ecological functions of Acidobacte